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| Status |
Public on Feb 06, 2019 |
| Title |
Genome-wide maps of chromatin state and gene expression in control and TPA-stimulated pre-B leukemic 697 line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Linker histone H1 has been correlated with transcriptional inhibition, but the mechanistic basis of the inhibition and its reversal during gene activation has remained enigmatic. We report that H1-compacted chromatin, reconstituted in vitro, blocks transcription by abrogating core histone modifications by p300 but not activator and p300 binding. Transcription from H1-bound chromatin is elicited by H1 chaperone NAP1, which is recruited in a gene-specific manner through direct interactions with activator-bound p300 that facilitate core histone acetylation (by p300) and concomitant eviction of H1 and H2A/H2B. An analysis in B cells confirms the strong dependency on NAP1-mediated H1 eviction for induction of the silent CD40 gene and further demonstrates that H1 eviction, seeded by activator-p300-NAP1 interactions, is propagated over a CTCF-demarcated region through a distinct mechanism that also involves NAP1. Our results confirm direct transcriptional inhibition by H1 and establish a gene-specific H1 eviction mechanism through an activator-p300-NAP1-H1 pathway.
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| Overall design |
Genome-wide binding/occupancy profiling of Histone H1 and CTCF by ChIP-seq and gene expression profiling by RNA-seq in 697 cells treated without or with TPA (12-O-tetradecanoylphorbol-13-acetate)
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| |
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| Contributor(s) |
Chen W, Shimada M, Roeder RG |
| Citation(s) |
30902546 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA178765 |
Transcriptional regulatory mechanisms in B cell development and leukemogenesis |
THE ROCKEFELLER UNIVERSITY |
ROBERT G ROEDER |
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| |
| Submission date |
Feb 05, 2019 |
| Last update date |
Feb 12, 2020 |
| Contact name |
Wei-Yi Chen |
| E-mail(s) |
chenwy@nycu.edu.tw
|
| Phone |
+886-2-2826-7328
|
| Organization name |
National Yang Ming Chiao Tung University
|
| Department |
Institute of Biochemistry and Molecular Biology
|
| Street address |
No. 155, Sec 2, Linong St.
|
| City |
Taipei |
| ZIP/Postal code |
112 |
| Country |
Taiwan |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA520988 |
| SRA |
SRP183668 |
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