|
| Status |
Public on Jan 21, 2021 |
| Title |
ATAC-seq profiles of B cells and naive and activated T cells from human peripheral blood |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Mature B cells, CD4+ T cells and CD8+ T cells were isolated from the peripheral blood of two human donors. Naive CD4+ and CD8+ T cells were cultured for three days in the presence of Dynabeads Human T-Activator CD3/CD28 beads (Life Technologies, cat. no. 111.31D) to induce activation. The B cells and naive and activated T cells were profiled by ATAC-seq to determine chromatin accessibility.
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| |
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| Overall design |
Cells were isolated from two human donors, resulting in two biological replicates of each combination of cell type and activation status.
|
| |
|
| Contributor(s) |
Bediaga NG, Smyth GK |
| Citation(s) |
33436846 |
| |
| Submission date |
Feb 05, 2019 |
| Last update date |
Jan 21, 2021 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (10)
|
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| This SubSeries is part of SuperSeries: |
| GSE126117 |
RNA-seq, ATAC-seq and Hi-C profiles of B cells and naïve and activated T cells from human peripheral blood |
|
| Relations |
| BioProject |
PRJNA521045 |
| SRA |
SRP183766 |
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