 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 03, 2008 |
| Title |
Expression data for a study on cross-species hybridization on single-species microarrays |
| Platform organism |
Xenopus laevis |
| Sample organisms |
Xenopus borealis; Xenopus laevis; Xenopus muelleri; Xenopus laevis x Xenopus borealis |
| Experiment type |
Expression profiling by array
Genome variation profiling by array
|
| Summary |
Gene expression was examined in testis and brain tissue between two species (Xenopus laevis and Xenopus borealis) and their hybrid.
Genomic DNA hybridizations of the microarray target and non-target species were used to select probes that are unbiased between species.
|
| |
|
| Overall design |
Gene expression:
3 biological replicates were used for each tissue type.
Genomic hybridization:
We used the ratio of probe gDNA hybridization intensities between the target and non-target species to create a probemask for expression data from these species.
Genomic DNA was extracted from blood samples of several Xenopus individuals. Xenopus laevis individuals were lab bread, Xenopus borealis individuals were from Kenya, and Xenopus muelleri individuals were from Swaziland.
|
| |
|
| Contributor(s) |
Chain FJ, Ilieva D, Evans BJ, Kelley DB |
| Citation(s) |
18815615 |
| |
| Submission date |
Aug 29, 2008 |
| Last update date |
Sep 20, 2012 |
| Contact name |
Frederic Chain |
| E-mail(s) |
chainfj@mcmaster.ca
|
| Organization name |
McMaster University
|
| Department |
Biology
|
| Lab |
LSB 324
|
| Street address |
1280 Main Street West
|
| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8S4K1 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL1318 |
[Xenopus_laevis] Affymetrix Xenopus laevis Genome Array |
|
| Samples (15)
|
| GSM316182 |
Xenopus borealis Brain, biological rep 1 |
| GSM316651 |
Xenopus borealis Brain, biological rep 2 |
| GSM316731 |
Xenopus borealis Brain, biological rep 3 |
| GSM316732 |
Xenopus borealis Testis, biological rep 1 |
| GSM316733 |
Xenopus borealis Testis, biological rep 2 |
| GSM316734 |
Xenopus borealis Testis, biological rep 3 |
| GSM316735 |
Xenopus laevis Brain, biological replicate 1 |
| GSM316736 |
Xenopus laevis Brain, biological replicate 2 |
| GSM316737 |
Xenopus laevis Brain, biological replicate 3 |
| GSM317609 |
Xenopus laevis genomic DNA |
| GSM317610 |
Xenopus borealis genomic DNA |
| GSM317611 |
Xenopus muelleri genomic DNA |
|
| Relations |
| BioProject |
PRJNA112793 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12625_RAW.tar |
58.1 Mb |
(http)(custom) |
TAR (of CEL, TXT) |
| GSE12625_genomic_hyb_readme.txt |
227 b |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...