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Series GSE126834 Query DataSets for GSE126834
Status Public on Mar 08, 2019
Title Single-cell analysis of homeostatic and regenerative adult skeletal muscle stem cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Skeletal muscle stem cells (MuSCs) ensure the formation and homeostasis of skeletal muscle and are responsible for its growth and repair processes. For repair to occur, MuSCs must exit from quiescence, abandon their niche and asymmetrically and symmetrically divide to reconstitute the stem cell pool and give rise to muscle progenitors, respectively. The transcriptomes of pooled MuSCs have provided a rich source of information for describing the genetic programs underlying distinct static cell states; however, bulk microarray and RNA-seq afford only averaged gene expression profiles, which blur the heterogeneity and developmental dynamics of asynchronous MuSC populations. The granularity required to identify distinct cell types, states, and their dynamics can be provided by single-cell analysis. We were able to compare the transcriptomes of thousands of MuSCs and primary myoblasts isolated from homeostatic or regenerating muscles by single-cell RNA- sequencing. Using computational approaches, we could reconstruct dynamic trajectories and place, in a pseudotemporal manner, the transcriptomes of individual MuSC within these trajectories. This approach allowed for the identification of distinct clusters of MuSCs and primary myoblasts with partially overlapping but distinct transcriptional signatures, as well as the description of metabolic pathways associated with defined MuSC states.
Overall design Single cells suspentions were loaded into 10X Genomics for single cells transcriptome analysis using 10 X Genomics single cells RNAseq kit v2.2
Contributor(s) Dell’Orso S, Juan AH, Ko K, Naz F, Gutierrez-Cruz G, Feng X, Sartorelli V
Citation(s) 30890574
Submission date Feb 20, 2019
Last update date Jun 07, 2019
Contact name Vittorio Sartorelli
Organization name NIH
Department NIAMS
Street address 50 South Dr.
City Bethsda
State/province MD
ZIP/Postal code 20814
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (7)
GSM3614992 total_muscle_wt_rep1
GSM3614993 total_muscle_wt_rep2
GSM3614994 homeostatic_MuSCs_rep1
BioProject PRJNA523432
SRA SRP186474

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Supplementary file Size Download File type/resource
GSE126834_RAW.tar 109.0 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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