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| Status |
Public on Mar 01, 2020 |
| Title |
RNAseq analysis of Arabis alpina buds in the dormant zone (V2) and buds in the growing zone (V3) at the end of vernalization (+0) and 5 days later (+5d) |
| Organism |
Arabis alpina |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Arabis alpina, similar to woody perennials, has a complex architecture with a zone of axillary vegetative branches and a zone of dormant buds that serve as perennating organs. We show that floral development during vernalization is the key for shaping the dormant bud zone by facilitating a synchronized and rapid growth after vernalization and thereby causing an increase in auxin transport, response and endogenous indole-3-acetic acid (IAA) levels in the stem. Floral development during vernalization is associated with the development of axillary buds in subapical nodes. Our transcriptome analysis indicated that these buds are not dormant during vernalization but only reach sustained growth after the return to warm temperatures. Floral and subapical vegetative branches grow after vernalization and inhibit the development of the buds below. Dormancy in these buds is regulated across the A. alpina life cycle by low temperatures and by apical dominance in a BRANCHED 1 dependent mechanism.
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| Overall design |
12 samples, 3 biological replicates per condition
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| Contributor(s) |
Vayssieres A, Mishra P, Albani MC |
| Citation(s) |
32022273 |
| |
| Submission date |
Feb 22, 2019 |
| Last update date |
Jul 24, 2020 |
| Contact name |
Maria Albani |
| Organization name |
University of Cologne
|
| Department |
Botanical institute
|
| Street address |
Carl-von-Linné-Weg 10
|
| City |
Köln |
| ZIP/Postal code |
50829 |
| Country |
Germany |
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| Platforms (1) |
| GPL26216 |
Illumina HiSeq 3000 (Arabis alpina) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA523826 |
| SRA |
SRP186602 |
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