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Series GSE127220 Query DataSets for GSE127220
Status Public on Oct 22, 2019
Title Sequencing of all RNA species by next generation sequencing of wildtype and GCNAKO Drosophila ovaries
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To identify significantly different individual mRNA species or genetic pathways in the GCNAKO mutant sample when compared to the wildtype sample. GCNA is encoded by CG14814 (Flybase ID: FBgn0023515).

Method: Total RNA was extracted using the Qiagen miRNEasy kit, followed by on column DNAse digest using the Qiagen DNAse digest kit. We used the miRNEasy kit to ensure collection of small RNA species as well as total RNA.

RNA was collected from three independent bological replicates of freshly dissected Drosophila ovaries ( 100 ovaries per replicate), collected from 1-3 day old female Drosophila. Ovaries were flash frozen in liquid nitrogen after dissection to preserve the RNA species and prevent degradation. RNAse free conditions were maintained throughout the extraction protocol. RNA quality was tested by the sequencing core before sequencing the RNA.
 
Overall design Whole trasncriptome or total RNA profiles were generated of 1-3 day old Drosophila ovaries by next generation sequencing using the Illumina HiSeq 2500 .
 
Contributor(s) Bhargava V, Buszczak M
Citation(s) 31839537
Submission date Feb 26, 2019
Last update date Jan 21, 2020
Contact name Michael Buszczak
E-mail(s) Michael.Buszczak@UTSouthwestern.edu
Phone 214-648-4941
Organization name University of Texas Southwestern Medical Center
Department Molecular Biology
Lab Buszczak Lab
Street address 5323 Harry Hines Boulevard
City Dallas
State/province Texas
ZIP/Postal code 75390
Country USA
 
Platforms (1)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (6)
GSM3631051 WT-1
GSM3631052 WT-2
GSM3631053 WT-3
Relations
BioProject PRJNA524361
SRA SRP186926

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127220_GCNA_wberlin_normalizedcounts.txt.gz 526.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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