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Series GSE127262 Query DataSets for GSE127262
Status Public on May 31, 2022
Title ZFP281-BRCA2 prevents R-loop accumulation during DNA replication
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary R-loops are prevalent in mammalian genomes and involved in many fundamental cellular processes. Depletion of BRCA2 leads to aberrant R-loop accumulation, contributing to genome instability. Here, we show that ZFP281 cooperates with BRCA2 in preventing R-loop accumulation to facilitate DNA replication in embryonic stem cells. Mechanistically, we demonstrate that ZFP281 can interact with BRCA2, and that BRCA2 is enriched at G/C-rich promoters and requires both ZFP281 and PRC2 for its proper recruitment to the bivalent chromatin at the genome-wide scale. Furthermore, depletion of ZFP281 or BRCA2 leads to accumulation of R-loops over the bivalent regions, and compromises activation of the developmental genes by retinoic acid during stem cell differentiation. In summary, our results reveal that ZFP281 recruits BRCA2 to the bivalent chromatin regions to ensure proper progression of DNA replication through preventing persistent R-loops.
Overall design ChIP-seq of BRCA2 in EED WT and KO ES cells. ChIP-seq of BRCA2 in mES cells after ZFP281 shRNA and non-targeting shRNA. ChIP-seq of BRCA2 in ZFP281 WT and KO ES cells.
Contributor(s) Liu X, Lin C
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Submission date Feb 27, 2019
Last update date Nov 29, 2022
Contact name Xiaoxu Liu
Organization name Southeast University
Department Institute of Life Sciences
Lab the Key Laboratory of Developmental Genes and Human Disease
Street address 2 Sipailou
City Nanjing
State/province Jiangsu
ZIP/Postal code 210018
Country China
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (9)
GSM3633348 ChIP-seq of BRCA2 in EED WT mESC
GSM3633349 EED WT mESC Input
GSM3664788 EED KO mESC Input
BioProject PRJNA524627
SRA SRP187030

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Supplementary file Size Download File type/resource
GSE127262_RAW.tar 1.2 Gb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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