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Series GSE127501 Query DataSets for GSE127501
Status Public on Apr 27, 2020
Title Effects of host miR-138 on herpes simplex virus 1 gene expression in Neuro-2a cells and their dependence of the binding sites on ICP0 mRNA
Organisms Mus musculus; Human alphaherpesvirus 1
Experiment type Expression profiling by high throughput sequencing
Summary We previously showed that miR-138 can repress herpes simplex virus 1 (HSV-1) ICP0 expression by binding to ICP0 mRNA. However, in this study we found that miR-138 can also repress viral gene expression independent of ICP0. Therefore we conducted this RNAseq experiment to assess the effects of miR-138 on expression of each individual viral gene to search for other viral targets of miR-138
Overall design We transfected Neuro-2a cell with a miR-138 mimic or a scrambled control, and then infected them with wild-type or M138 mutant virus (which contains mutations in the miR-138 binding sites in ICP0 mRNA). For both wild-type and mutant viruses, miR-138 transfected cells were compared with scrambled control transfected cells. Three biological replicates for each condition.
Contributor(s) Pan D, Coen DM
Citation(s) 33558653
Submission date Feb 28, 2019
Last update date Mar 16, 2021
Contact name Dongli Pan
Organization name Zhejiang University
Street address 866 Yuhangtang Road
City Hangzhou
State/province Zhejiang
ZIP/Postal code 110058
Country China
Platforms (1)
GPL26237 Illumina NextSeq 500 (Human alphaherpesvirus 1; Mus musculus)
Samples (12)
GSM3635600 Control_WT_1
GSM3635601 Control_WT_2
GSM3635602 Control_WT_3
This SubSeries is part of SuperSeries:
GSE127504 Identification of herpes simplex virus 1 and host targets of miR-138 by combined RNAseq/PARCLIP
BioProject PRJNA524882
SRA SRP187092

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127501_RAW.tar 20.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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