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| Status |
Public on Apr 27, 2020 |
| Title |
Identification of targets of miR-138 in Neuro-2a and 293T cells by RNAseq |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We previously showed that miR-138 can repress herpes simplex virus 1 (HSV-1) ICP0 expression by binding to ICP0 mRNA. However, in this study we found that miR-138 can also repress viral gene expression independent of ICP0. We did not find other confirmed viral targets of miR-138. Therefore we conducted these RNAseq experiments (in combination with PAR-CLIP experiments whose results are uploaded separately) to identify host targets of miR-138 in two cell lines to explain the ICP0-independent effects on HSV-1 gene expression.
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| Overall design |
We transfected Neuro-2a cells or 293T cells with a miR-138 mimic or a scrambled control mimic, and compared miR-138 transfected with control transfected cells. Three biological replicates for each condition.
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| Contributor(s) |
Pan D, Coen DM |
| Citation(s) |
33558653 |
| |
| Submission date |
Feb 28, 2019 |
| Last update date |
Mar 16, 2021 |
| Contact name |
Dongli Pan |
| E-mail(s) |
pandongli@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Street address |
866 Yuhangtang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
110058 |
| Country |
China |
| |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE127504 |
Identification of herpes simplex virus 1 and host targets of miR-138 by combined RNAseq/PARCLIP |
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| Relations |
| BioProject |
PRJNA524883 |
| SRA |
SRP187090 |
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