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Series GSE127798 Query DataSets for GSE127798
Status Public on Oct 01, 2023
Title The effect of BMP4/6 stimulation on adult muscle stem cells (MuSC)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Muscle development and regeneration strongly depends on extracellular cues and growth factors, which are taken up by muscle stem cells and guide them either towards proliferation and fusion or towards quiescence. Here we investigated the effect of bone morphogenetic protein (BMP) signaling on Pax7 positive adult muscle stem cells of the mouse.
We discovered a cross talk between the BMP- and NOTCH-signalling pathways, leading to high upregulation upon BMP4/BMP6-stimulation of Hes1 mRNA which is a key component of the NOTCH-siganling pathway.
Overall design Muscle stem cells were isolated from muscle of female 6-8-week-old mice, cultured on Matrigel ® for expansion over 2 days. In order to limit the effect of BMPs in the culture medium (e.g. from serum supplements), cells were cultured prior to the BMP stimulation for 6 h in serum-free medium containing BMP soluble receptor (recombinant mouse BMPR-IA/ALK3-Fc Chimera, R&D Systems, 437-MR) in a concentration of 200 ng/ml. Cells were then stimulated with a pulse of 1 h with 100 ng/ml BMP4 or BMP6 or only with vehicle used for reconstitution of BMP in the case of controls. Cells were harvested thereafter and total RNA was extracted.
Contributor(s) Polydorou I, Malatras A, Schuelke M, Amthor H
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Submission date Mar 04, 2019
Last update date Oct 01, 2023
Contact name Markus Schuelke
Phone ++49 30 4505 66112
Organization name Charite
Department Neuropediatrics
Lab Schuelke lab
Street address Augustenburger Platz 1
City Berlin
State/province Berlin
ZIP/Postal code 13353
Country Germany
Platforms (1)
GPL26250 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENSG v.21.0.0]
Samples (6)
GSM3639086 MuSC BMP6 treated, biological rep. 1
GSM3639087 MuSC BMP6 treated, biological rep. 2
GSM3639088 MuSC BMP6 treated, biological rep. 3
BioProject PRJNA525422

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Supplementary file Size Download File type/resource
GSE127798_RAW.tar 48.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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