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| Status |
Public on Oct 01, 2023 |
| Title |
The effect of BMP4/6 stimulation on adult muscle stem cells (MuSC) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Muscle development and regeneration strongly depends on extracellular cues and growth factors, which are taken up by muscle stem cells and guide them either towards proliferation and fusion or towards quiescence. Here we investigated the effect of bone morphogenetic protein (BMP) signaling on Pax7 positive adult muscle stem cells of the mouse.
We discovered a cross talk between the BMP- and NOTCH-signalling pathways, leading to high upregulation upon BMP4/BMP6-stimulation of Hes1 mRNA which is a key component of the NOTCH-siganling pathway.
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| Overall design |
Muscle stem cells were isolated from muscle of female 6-8-week-old mice, cultured on Matrigel ® for expansion over 2 days. In order to limit the effect of BMPs in the culture medium (e.g. from serum supplements), cells were cultured prior to the BMP stimulation for 6 h in serum-free medium containing BMP soluble receptor (recombinant mouse BMPR-IA/ALK3-Fc Chimera, R&D Systems, 437-MR) in a concentration of 200 ng/ml. Cells were then stimulated with a pulse of 1 h with 100 ng/ml BMP4 or BMP6 or only with vehicle used for reconstitution of BMP in the case of controls. Cells were harvested thereafter and total RNA was extracted.
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| Contributor(s) |
Polydorou I, Malatras A, Schuelke M, Amthor H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 04, 2019 |
| Last update date |
Oct 01, 2023 |
| Contact name |
Markus Schuelke |
| E-mail(s) |
markus.schuelke@charite.de
|
| Phone |
++49 30 4505 66112
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| Organization name |
Charite
|
| Department |
Neuropediatrics
|
| Lab |
Schuelke lab
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
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| Platforms (1) |
| GPL26250 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENSG v.21.0.0] |
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| Samples (6)
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| GSM3639089 |
MuSC vehicle treated, biological rep. 1 |
| GSM3639090 |
MuSC vehicle treated, biological rep. 2 |
| GSM3639091 |
MuSC vehilce treated, biological rep. 3 |
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| Relations |
| BioProject |
PRJNA525422 |
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