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Series GSE128498 Query DataSets for GSE128498
Status Public on Sep 04, 2019
Title Arabidopsis PP6 phosphatases dephosphorylate PIF proteins to repress photomorphogenesis.
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Light-induced phosphorylation is necessary and essential for the degradation of phytochrome-interacting factors (PIFs), the central repressors of photomorphogenesis. Although the kinases responsible for PIF phosphorylation have been extensively studied, the phosphatases underlying PIF dephosphorylation are largely unknown. Here, we real that mutation of FyPP1 and FyPP3, two catalytic subunits of PP6 phosphatases, promoted photomorphogenesis of seedlings in the dark. PP6 and PIFs functioned synergistically to repress photomorphogenesis. FyPP1 and FyPP3 directly interacted with and dephosphorylated PIF3 and PIF4. The light-induced degradation of PIF4 and the PIF transcriptional activities were dependent on PP6 activity. These data demonstrate that PP6 phosphatases repress photomorphogenesis through regulation of PIF phosphorylation, protein stability and transcriptional activity.
Overall design RNA-seq to arabidopsis thaliana seedlings of Col,f1f3 and pifq, grown in the dark and at 22°C for 4 days. Each sample has 2 biological replicates.
Contributor(s) Yu X
Citation(s) 31527236
Submission date Mar 18, 2019
Last update date Dec 04, 2019
Contact name xiaodan yu
Phone 13476069559
Organization name Huazhong Agricultural University
Department College of Life Science and Technology
Street address No.1,Shizishan Street · Hongshan District
City Wuhan
State/province Hubei Province
ZIP/Postal code 430070
Country China
Platforms (1)
GPL17639 Illumina HiSeq 2500 (Arabidopsis thaliana)
Samples (6)
GSM3677733 COL_rep1
GSM3677734 COL_rep2
GSM3677735 f1f3_rep1
BioProject PRJNA527851
SRA SRP188768

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Supplementary file Size Download File type/resource
GSE128498_RAW.tar 680.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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