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Series GSE128724 Query DataSets for GSE128724
Status Public on Aug 01, 2019
Title Gene expression profiling of Trop2+ and Trop2- luminal epithelial cells and stromal cells from young adult and old mouse prostates
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We set out to determine if distinct prostate luminal epithelial cell-types exhibited age-related differences in their gene expression profiles. We used fluorescence activated cell sorting to isolate Trop2+ and Trop2- luminal cells and EpCAM- CD49f- stromal cells from dissociated preparations of prostate tissue taken from young adult (3 months) and old (24 months of age) mice. RNA was extracted from sorted cells to evaluate differences in gene expression.
 
Overall design RNA sequencing of Trop2+ and Trop2- luminal cells and stromal cells isolated from 3 month and 24 month old mouse prostates. Experiments were performed using biological triplicates for stromal cells and duplicates for luminal subsets. Sequencing was performed on Illumina HiSeq 3000.
 
Contributor(s) Goldstein AS
Citation(s) 31390564, 32929410
Submission date Mar 22, 2019
Last update date Sep 21, 2020
Contact name Andrew Scott Goldstein
Organization name UCLA
Department Urology
Lab Goldstein
Street address 610 Charles E Young Dr East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (14)
GSM3683710 Stromal Old Rep 1
GSM3683711 Stromal Old Rep 2
GSM3683712 Stromal Old Rep 3
Relations
BioProject PRJNA528642
SRA SRP189170

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128724_RAW.tar 6.2 Mb (http)(custom) TAR (of TXT)
GSE128724_TMM_normalized_reads_count_per_million.xlsx 3.8 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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