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Series GSE129014 Query DataSets for GSE129014
Status Public on Nov 22, 2019
Title Automated High Frequency Sampling of Stem Cell Differentiation Reveals Early Stage Divergence of Human and Mouse Gene Expression Kinetics
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed whole transcriptome RNA sequencing experiments of neural differentiation time courses starting from human ES cells (EGFP-H1) and mouse EGFP-EpiS cells. Cells were sampled at high temporal frequency using an automated Tecan Tissue Culture robotics system to sample mouse cells every 4 mins and human cells every 10 mins for the first 10 hours of neural differentiation.
 
Overall design Cells were sampled at high temporal frequency using an automated Tecan Tissue Culture robotics system to sample mouse cells every 4 mins and human cells every 10 mins for the first 10 hours of neural differentiation. Cells were pre-seeded in pluripotent conditions (E8 on matrigel for H1 cells and TSS on matrigel for mEpiS cells), then were switched to neural differentiation media and immediately sampled every 4 or 10 mins for mouse and human cells, respectively, for 10 hours.
 
Contributor(s) Barry C, Schmitz MT, Argus C, Bolin JM, Probasco MD, Leng N, Duffin BM, Steill J, Swanson SA, McIntosh BE, Stewart R, Kendziorski C, Thomson JA, Bacher R
Citation(s) 31815944
Submission date Mar 28, 2019
Last update date Feb 24, 2020
Contact name Rhonda Bacher
E-mail(s) rbacher@ufl.edu
Organization name University of Florida
Street address 2004 Mowry Rd.
City Gainesville
State/province FL
ZIP/Postal code 32603
Country USA
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (327)
GSM3690335 E8_0_038911_0023279
GSM3690336 E8_0_038911_0039788
GSM3690337 NBplusNog_10min_038914_0023282
Relations
BioProject PRJNA529707
SRA SRP189795

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE129014_hg19genes.no_mt.ec.tab.gz 5.7 Mb (ftp)(http) TAB
GSE129014_hg19genes.no_mt.tpm.rescale.tab.gz 5.9 Mb (ftp)(http) TAB
GSE129014_mm10genes.no_mt.ec.tab.gz 4.2 Mb (ftp)(http) TAB
GSE129014_mm10genes.no_mt.tpm.rescale.tab.gz 4.5 Mb (ftp)(http) TAB
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Raw data are available in SRA
Processed data are available on Series record

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