GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE129016 Query DataSets for GSE129016
Status Public on May 07, 2019
Title Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary This research was undertaken to investigate the global role of inositol phosphorylceramide synthase (IPCS) activity in plants and reveal its potential as a herbicide target. The non-mammalian enzyme is a key component in the plant sphingolipid biosynthetic pathway and is shown here to be a possible herbicide target. RNA-Seq analyses demonstrated that over-expression of inositol phosphorylceramide synthase isoforms AtIPCS1, 2 or 3 in Arabidopsis thaliana resulted in the down-regulation of genes involved in plant response to pathogens. In addition, genes associated with the abiotic stress response to salinity, cold and drought were found to be similarly down-regulated. Detailed analyses of transgenic lines over-expressing AtIPCS1-3 at various levels revealed that the degree of down-regulation is specifically correlated with the level of IPCS expression. Singular enrichment analysis of these down-regulated genes showed that AtIPCS1-3 expression affects biological signaling pathways involved in plant response to biotic and abiotic stress. The up-regulation of genes involved in photosynthesis and lipid localization was also observed in the over-expressing lines.
Overall design Two biological replicates were analyzed for each AtPCS isoform (three IPCS isoforms; two levels of AtIPCS expression per isoform)
Contributor(s) Pinneh E, Denny P
Citation(s) 31120963
Submission date Mar 28, 2019
Last update date Jun 21, 2019
Contact name Paul William Denny
Phone 01913343983
Organization name Durham University
Department Biosciences
Street address Lower Mountjoy
City Durham
State/province Durham
ZIP/Postal code DH1 3LE
Country United Kingdom
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (21)
GSM3690668 At1+_3-1
GSM3690669 At1+_3-2
GSM3690670 At1+_3-3
BioProject PRJNA529715
SRA SRP189805

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129016_Pinneh_GEO_processed.txt.gz 2.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap