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| Status |
Public on May 07, 2019 |
| Title |
Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This research was undertaken to investigate the global role of inositol phosphorylceramide synthase (IPCS) activity in plants and reveal its potential as a herbicide target. The non-mammalian enzyme is a key component in the plant sphingolipid biosynthetic pathway and is shown here to be a possible herbicide target. RNA-Seq analyses demonstrated that over-expression of inositol phosphorylceramide synthase isoforms AtIPCS1, 2 or 3 in Arabidopsis thaliana resulted in the down-regulation of genes involved in plant response to pathogens. In addition, genes associated with the abiotic stress response to salinity, cold and drought were found to be similarly down-regulated. Detailed analyses of transgenic lines over-expressing AtIPCS1-3 at various levels revealed that the degree of down-regulation is specifically correlated with the level of IPCS expression. Singular enrichment analysis of these down-regulated genes showed that AtIPCS1-3 expression affects biological signaling pathways involved in plant response to biotic and abiotic stress. The up-regulation of genes involved in photosynthesis and lipid localization was also observed in the over-expressing lines.
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| Overall design |
Two biological replicates were analyzed for each AtPCS isoform (three IPCS isoforms; two levels of AtIPCS expression per isoform)
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| Contributor(s) |
Pinneh E, Denny P |
| Citation(s) |
31120963 |
| |
| Submission date |
Mar 28, 2019 |
| Last update date |
Jun 21, 2019 |
| Contact name |
Paul William Denny |
| E-mail(s) |
p.w.denny@durham.ac.uk
|
| Phone |
01913343983
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| Organization name |
Durham University
|
| Department |
Biosciences
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| Street address |
Lower Mountjoy
|
| City |
Durham |
| State/province |
Durham |
| ZIP/Postal code |
DH1 3LE |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA529715 |
| SRA |
SRP189805 |
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