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Series GSE129230 Query DataSets for GSE129230
Status Public on Oct 22, 2019
Title GR cistromes reprogramming by High Fat Diet [ChIP-seq H3K27ac]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We mapped the genome-wide profiles of Histone H3K27 acetylation (two time points, ZT0 and ZT12) by using ChIP-Seq in livers from control mice and mice fed High Fat Diet (HFD) for 12 weeks
Overall design C57BL/6J mice were fed High Fat Diet (Research Diet, D12331) and control diet (Research Diet, D12329) for 12 weeks. Mice were sacrificed by cervical dislocation at ZT0 (6 am) and at ZT12 (6 pm), liver collected and snap frozen. ChIP was performed as previously described (Uhlenhaut et al. 2012). Sheared chromatin was immunoprecipitated with anti-H3K27ac antibody (Abcam, ab4729). Two replicates per condition and time point were used, for a total of 8 ChIP-seq samples.
Contributor(s) Mir AA, Quagliarini F
Citation(s) 31706703
Submission date Apr 02, 2019
Last update date Jan 21, 2020
Contact name Nina Henriette Uhlenhaut
Organization name HelmholtzZentrum München - IDC
Department Molecular Endocrinology
Lab Uhlenhaut IDC
Street address Ingolstädter Landstraße 1, Neuherberg
City Munich
State/province Bavaria
ZIP/Postal code D-85764
Country Germany
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM3703240 H3K27ac_ZT0 control REP1
GSM3703241 H3K27ac_ZT0 control REP2
GSM3703242 H3K27ac_ZT0 HFD REP1
This SubSeries is part of SuperSeries:
GSE108690 Cistromic reprogramming of the diurnal glucocorticoid hormone response by high fat diet
BioProject PRJNA530560
SRA SRP190188

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Supplementary file Size Download File type/resource
GSE129230_RAW.tar 22.2 Mb (http)(custom) TAR (of BROADPEAK, GAPPEDPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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