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| Status |
Public on Oct 22, 2019 |
| Title |
GR cistromes reprogramming by High Fat Diet [ChIP-seq H3K27ac] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We mapped the genome-wide profiles of Histone H3K27 acetylation (two time points, ZT0 and ZT12) by using ChIP-Seq in livers from control mice and mice fed High Fat Diet (HFD) for 12 weeks
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| Overall design |
C57BL/6J mice were fed High Fat Diet (Research Diet, D12331) and control diet (Research Diet, D12329) for 12 weeks. Mice were sacrificed by cervical dislocation at ZT0 (6 am) and at ZT12 (6 pm), liver collected and snap frozen. ChIP was performed as previously described (Uhlenhaut et al. 2012). Sheared chromatin was immunoprecipitated with anti-H3K27ac antibody (Abcam, ab4729). Two replicates per condition and time point were used, for a total of 8 ChIP-seq samples.
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| Contributor(s) |
Mir AA, Quagliarini F |
| Citation(s) |
31706703 |
| |
| Submission date |
Apr 02, 2019 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Nina Henriette Uhlenhaut |
| E-mail(s) |
henriette.uhlenhaut@helmholtz-muenchen.de
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| Organization name |
HelmholtzZentrum München - IDC
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| Department |
Molecular Endocrinology
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| Lab |
Uhlenhaut IDC
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| Street address |
Ingolstädter Landstraße 1, Neuherberg
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| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
D-85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE108690 |
Cistromic reprogramming of the diurnal glucocorticoid hormone response by high fat diet |
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| Relations |
| BioProject |
PRJNA530560 |
| SRA |
SRP190188 |
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