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| Status |
Public on Mar 24, 2009 |
| Title |
PPARg dependent expression changes during differentiation of 3T3L1 cell into adipocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We profiled PPARg dependent gene expression changes during differntiation of 3T3L1 cell using PPARg siRNA
3T3-L1 (Pre-adipocyte) cell line was induced to differentiate using standard adipocyte differentiation media (IBMX, Dex and Insulin) 48hrs post-confluency. RNA was harvested at day -2 (confluent fibroblasts), 48hrs post-induction with IBMX, DEX and Insulin (day=0) and for each subsequent day after rosiglitazone treatment. Illumina beadchip microarrays were used to determine expression profiles of genes differentially regulated in cells transfected with either siRNA targeting PPARgamma or a non-targeting control siRNA.
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| Overall design |
3T3L1 cell were induced to differentiate into adipocytes using IBMX, DEX and Insulin. RNA from cell treated with PPARg-specific siRNA and non-specific siRNA was isolated at different timepoints. Illumina MouseRef-8 v1.1 Bead chips were used for expression profiling
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| |
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| Contributor(s) |
Hamza MS, Pott S, Vega VB, Thomsen JS, Kandhadayar GS, Chiu KP, Wei CL, Ruan Y, Liu ET |
| Citation(s) |
19300518 |
| |
| Submission date |
Sep 25, 2008 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Vinsensius B Vega |
| E-mail(s) |
vegav@gis.a-star.edu.sg
|
| Organization name |
Genome Institute of Singapore
|
| Street address |
60 Biopolis Street
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL6103 |
Illumina mouseRef-8 v1.1 expression beadchip |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA110849 |
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