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Series GSE12943 Query DataSets for GSE12943
Status Public on Dec 01, 2008
Title Profiling antibody function on antigen arrays
Organism Homo sapiens
Experiment type Protein profiling by protein array
Summary Humoral immune responses are traditionally characterized by determining the presence and quality of antibodies specific for certain antigens. Arraying of large numbers of antigens allows the parallel measurement of antibodies, generating patterns called antibody profiles. Functional characterization of these antibodies could help draw an even more informative map of an immune response. To generate functional antibody profiles we simultaneously tested not only IgM, IgG and IgA binding to but also complement activation by a panel of endogenous and exogenous antigens printed as microarrays, using normal and autoimmune human sera. We show that complement activation by a particular antigen in a given individual cannot be predicted by the measurement of antigen specific antibodies, in spite of a general correlation between the amount of antigen-bound antibody and the deposited C3 fragments. This is due to both differences in the isotypes that dominate in the recognition of an antigen and individual variations for a given isotype, resulting in altered complement activation potential. Thus, antigen specific C3 deposition can be used as an additional parameter in immune response monitoring. This is exemplified by comparing the coordinates of antigens, used for the diagnosis of systemic lupus erythematosus, of normal and autoimmune serum samples in a two-dimensional space derived from C3 deposition and antibody binding. Since cleavage fragments of C3 mediate important immunological processes we propose that measurement of their deposition on antigen microarrays, in addition to antibody profiling, can provide useful functional signature about the tested serum.

Keywords: IgM immuneprofile, antigen array
Overall design IgM, IgG, IgA and C3 binding in 30 human serum samples were examined using custom-made protein arrays
Contributor(s) Papp K, Végh P, Prechl J
Citation(s) 19018009
Submission date Sep 26, 2008
Last update date Mar 20, 2012
Contact name József Prechl
Phone +3613812175
Fax +3613812176
Organization name ELTE-MTA
Department Immunology Research Group
Lab Immunoproteomics
Street address Pázmány Péter s. 1/C
City Budapest
ZIP/Postal code 1117
Country Hungary
Platforms (1)
GPL7390 AbC ELTE_human_960_v1
Samples (120)
GSM324586 Patient_#1000_IgM
GSM324587 Patient_#2000_IgM
GSM324588 Patient_#2988_IgM
BioProject PRJNA110967

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12943_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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