|
| Status |
Public on Apr 07, 2019 |
| Title |
Gene expression of zebrafish larvae upon treating with lalistat |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To test if HIF pathway is regulated by lipids in zebrafish larvae, we have employed whole genome microarray expression profiling as a discovery platform to identify induced genes upon lalistat treatment. Lalistat is a specific inhibitor for lysosomal acid lipase (LAL), which is required for break down triglyceride or cholesterol ester to release free fatty acids.
|
| |
|
| Overall design |
Zebrafish larvae (5dpf) were treated with vehicle control DMSO or lalistat (10uM) for 2hr. Each RNA sample were extracted from pooled 4 larvae. Two biological replicates (2 clutches from different pairs of WT zebrafish mating) were conducted.
|
| |
|
| Contributor(s) |
Shao W, Espenshade P |
| Citation(s) |
33109611 |
| |
| Submission date |
Apr 06, 2019 |
| Last update date |
Jun 15, 2021 |
| Contact name |
Wei Shao |
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Cell Biology
|
| Lab |
Peter Espenshade
|
| Street address |
725 N Wolfe Street
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14688 |
Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Feature Number version) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA531275 |
Less...
More...