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Series GSE129532 Query DataSets for GSE129532
Status Public on Sep 01, 2020
Title Florigen family chromatin recruitment, competition and targets [ChIP-Seq]
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary This study identified direct targets of TFL1 and FD and showed that TFL1 is recruited to the chromatin by FD. We performed ChIP-seq using anti-GFP antibodies in gTFL1-GFP tfl1-1, gTFL1-GFP fd-1 and controls (nontransgenic plants). We used anti-GUS antibodies for gFD-GUS fd-1. ChIP was conducted on dissected, non-bolted, inflorescences from 42-day-old short-day-grown Arabidopsis thaliana.
 
Overall design Identification of binding sites for FD and TFL1 in axillary buds in wildtype Arabidopsis Col-0 short-day grown plants. Two biological ChIP replicates were sequence for FD fd and TFL1 fd, four for TFL1 tfl1; two anti GFP mock (controls) each for wild type and fd. Four inputs were sequenced.
 
Contributor(s) Klasfeld S, Zhu Y, Wagner D
Citation(s) 33046692
https://onlinelibrary.wiley.com/doi/full/10.1111/resp.13557
Submission date Apr 09, 2019
Last update date Oct 06, 2021
Contact name Doris Wagner
E-mail(s) wagnerdo@sas.upenn.edu
Phone 2158980483
Organization name University of Pennsylvania
Department Department of Biology
Lab Wagner Lab
Street address 103G Carolyn Lynch Laboratory
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (16)
GSM4215572 Shoot apices, gFD_GUS Rep1 ChIP-Seq
GSM4215573 Shoot apices, gFD_GUS Rep2 ChIP-Seq
GSM4215574 Shoot apices, gTFL1_GFP A Rep1 ChIP-Seq
This SubSeries is part of SuperSeries:
GSE141894 Florigen family chromatin recruitment, competition and targets
Relations
BioProject PRJNA531668
SRA SRP191553

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129532_Control_for_fd_gTFL1_GFP_merged.bw 42.4 Mb (ftp)(http) BW
GSE129532_Control_merged.bigwig 91.3 Mb (ftp)(http) BIGWIG
GSE129532_RAW.tar 1.1 Gb (http)(custom) TAR (of BW)
GSE129532_all_input_merged.bigwig 396.5 Mb (ftp)(http) BIGWIG
GSE129532_fd_gTFL1_GFP_merged.bigwig 174.1 Mb (ftp)(http) BIGWIG
GSE129532_fd_gTFL1_GFP_qval10_peaks.narrowPeak.gz 302 b (ftp)(http) NARROWPEAK
GSE129532_gFD_GUS_merged.bigwig 179.4 Mb (ftp)(http) BIGWIG
GSE129532_gFD_GUS_qval10_peaks.narrowPeak.gz 109.4 Kb (ftp)(http) NARROWPEAK
GSE129532_gTFL1_GFP_merged.bigwig 316.9 Mb (ftp)(http) BIGWIG
GSE129532_gTFL1_GFP_qval10_peaks.narrowPeak.gz 82.7 Kb (ftp)(http) NARROWPEAK
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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