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Series GSE129726 Query DataSets for GSE129726
Status Public on Sep 02, 2019
Title Hematopoietic stem cells in perisinusoidal niches are protected from ageing [young and aged BM CD45-CD31+ endothelial cells]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary With ageing, intrinsic hematopoietic stem cell (HSC) activity decreases, resulting in impaired tissue homeostasis, reduced engraftment following transplantation and increased susceptibility to diseases. However, whether ageing affects also the HSC niche impairing the capacity to support HSC function is still largely unknown.
Here, by using in-vivo long-term label retention assays we demonstrate that aged labelling retaining (LR) HSCs, which are in the old mice the most quiescent HSC subpopulation with the highest regenerative capacity and cellular polarity, reside predominantly in perisinusoidal niches. Furthermore, we demonstrate that sinusoidal niches and perisinusoidal Nes-GFPlow cells are uniquely preserved in shape, morphology and number upon ageing. Finally, we show that myeloablative chemotherapy can selectively disrupt aged sinusoidal niches, which is linked to hematopoietic failure and decreased survival of aged mice.
Overall, our data characterize for the first time the functional alterations of the aged HSC niche and unveil that perisinusoidal niches are uniquely preserved and protect HSCs from ageing.
Overall design Single-cell RNA-Seq dataset of young and aged BM CD45-CD31+ endothelial cells. A total of five libraries was generated in three biological replicates (three libraries from young animals, two libraries from aged animals). All libraries were prepared using the 10X Genomics Chromium Single Cell 3’ Solution platform.
Contributor(s) Pospiech J
Citation(s) 31685996
Submission date Apr 12, 2019
Last update date Dec 03, 2019
Contact name Johannes Pospiech
Organization name Ulm University
Street address Albert-Einstein Allee 11
City Ulm
ZIP/Postal code 89081
Country Germany
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (5)
GSM3720878 young_CD31_1
GSM3720879 young_CD31_2
GSM3720880 young_CD31_3
This SubSeries is part of SuperSeries:
GSE130299 Hematopoietic stem cells in perisinusoidal niches are protected from ageing
BioProject PRJNA532599
SRA SRP192373

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129726_RAW.tar 5.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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