|
| Status |
Public on Feb 04, 2021 |
| Title |
Expression data from intestine samples of mice lacking intestinal Pcyt1a, which encodes an enzyme that reguates phosphatidylcholine synthesis. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Phosphatidylcholine is a membrane lipid. To determine its role in the intestine, we deleted the gene that produces this lipid specificallly in intestinal epithelial cells and conducted expression analysis.
We used microarray analysis to determine changes in gene expression that occur in the absence of intestinal phosphatidylcholine synthesis.
|
| |
|
| Overall design |
Mice lacking intestinal Pcyt1a and their Pcyt1a-positive controls were fasted overnight and re-fed a high fat diet. We then extracted RNA and hybridized to Affymetrix microarrays. Samples were collected from the geometric middle of the small intestine (jejunum).
|
| |
|
| Contributor(s) |
Jacobs RL, Kennelly JP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 14, 2019 |
| Last update date |
Feb 07, 2021 |
| Contact name |
René L Jacobs |
| E-mail(s) |
rjacobs@ualberta.ca
|
| Organization name |
University of Alberta
|
| Department |
Agriculture, Food and Nutritional Science; Biochemistry
|
| Lab |
Jacobs Lab
|
| Street address |
Li Ka Shing Centre for Health Research
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G2E1 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA532759 |
Less...
More...