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Series GSE130186 Query DataSets for GSE130186
Status Public on Jan 16, 2020
Title Selective egg cell polyspermy bypasses the triploid block
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Polyploidization, the increase in genome copies, is considered a major driving force for speciation. We have recently provided the first direct in planta evidence for polyspermy induced polyploidization. Capitalizing on a novel sco1-based polyspermy assay, we here show that polyspermy can selectively polyploidize the egg cell, while rendering the genome size of the ploidy-sensitive central cell unaffected. This unprecedented result indicates that polyspermy can bypass the triploid block, which is an established postzygotic polyploidization barrier. In fact, we here show that most polyspermy-derived seeds are insensitive to the triploid block suppressor admetos. The robustness of polyspermy-derived plants is evidenced by the first transcript profiling of triparental plants and our observation that these idiosyncratic organisms segregate tetraploid offspring within a single generation. Polyspermy-derived triparental plants are thus comparable to triploids recovered from interploidy crosses. Our results expand current polyploidization concepts and have important implications for plant breeding.
Overall design The dataset contains samples from biparental diploid, biparental triploid, and triparental triploid polyspermy plants. Each sample Is represented by five biological replicates and each biological replicate is represented by four technical replicates.
Sequencing performed by: Bielefeld University, CeBiTec & Faculty of Biology
Contributor(s) Mao Y, Gabel A, Nakel T, Viehöver P, Baum T, Tekleyohansm DG, Vo D, Grosse I, Groß-Hardt R
Citation(s) 32027307
Submission date Apr 23, 2019
Last update date Feb 18, 2020
Contact name Alexander Gabel
Organization name Helmholtz Institute for RNA-based Infection Research
Lab LncRNA and Infection Biology (LRIB)
Street address Josef-Schneider-Str. 2 / D15
City Würzburg
ZIP/Postal code 97080
Country Germany
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (60)
GSM3733546 BD01_01: Biparental diploid bio rep1 tech rep1
GSM3733547 BD01_02: Biparental diploid bio rep1 tech rep2
GSM3733548 BD01_03: Biparental diploid bio rep1 tech rep3
BioProject PRJNA534240
SRA SRP193460

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130186_deseq2_norm_counts.csv.gz 2.2 Mb (ftp)(http) CSV
GSE130186_deseq2_rlog_counts.csv.gz 2.5 Mb (ftp)(http) CSV
GSE130186_tpm.csv.gz 2.6 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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