NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE130274 Query DataSets for GSE130274
Status Public on Oct 09, 2019
Title Interdependent photo- and chemosensory systems regulate larval settlement in a marine sponge
Organism Amphimedon queenslandica
Experiment type Expression profiling by high throughput sequencing
Summary Marine pelagic larvae from throughout the animal kingdom use a hierarchy of environmental cues to identify a suitable benthic habitat on which to settle and metamorphose into the reproductive phase of the life cycle. The majority of larvae are induced to settle by biochemical cues and many species have long been known to preferentially settle in the dark. Combined, these data suggest that larval responses to light and biochemical cues may be linked, but this is yet to be explored at the molecular level. Here, we track vertical position of larvae of the sponge Amphimedon queenslandica to show that they descend to the benthos at twilight, by which time they are competent to respond to biochemical cues, consistent with them naturally settling in the dark. We then conduct larval settlement assays under three different light regimes (natural day-night, constant dark or constant light), and use transcriptomics on individual larvae to identify candidate molecular pathways underlying the different settlement responses that we observe. We find that constant light prevents larval settlement in response to biochemical cues, likely via actively repressing chemostransduction; this is consistent with the sustained upregulation of a photosensory cryptochrome and two putative inactivators of G-protein signalling in the constant light only. We hypothesise that photo- and chemosensory systems may be hierarchically integrated into ontogeny to regulate larval settlement via nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) signalling in this sponge that belongs to one of the earliest branching of the extant animal lineages.
 
Overall design Transcriptomic data for 43 individual larvae of the sponge Amphimedon queenslandica aged 1 and 9 hours post emergence (hpe) that were exposed to one of three experimental light regimes. Larvae were collected within a 30-minute window beginning at 11 am and were distributed into 0.22 μm filtered sea water, under one of three experimental light regimes, by 0.5 hours post emergence (hpe). The light regimes were (1) natural day-night, (2) constant dark (using LEE filter model 299, 1.2 neutral density, allowing 6.6% transmission of ambient daylight), and (3) constant light (full spectrum Lumilux T5 HE tube). For RNA-sequencing, two timepoints (1 and 9 hpe) were chosen to represent precompetent and competent larvae, respectively.
 
Contributor(s) Say TE, Degnan SM
Citation(s) 31791111
Submission date Apr 24, 2019
Last update date Jan 08, 2020
Contact name Tahsha Say
E-mail(s) say.tahsha@gmail.com
Organization name The University of Queensland
Department School of Biological Sciences
Lab Marine Genomics Lab
Street address The University of Queensland
City Brisbane
State/province QLD
ZIP/Postal code 4072
Country Australia
 
Platforms (1)
GPL26567 Illumina HiSeq 2500 (Amphimedon queenslandica)
Samples (43)
GSM3735026 larva_1hpe_constant_dark1_08
GSM3735027 larva_1hpe_constant_dark2_09
GSM3735028 larva_1hpe_constant_dark3_10
Relations
BioProject PRJNA534516
SRA SRP193781

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130274_TS1015_L001_L002_counting_report_tidy_NoERCC_NoBact_2016.02.08_renamed_12Apr19.txt.gz 1.3 Mb (ftp)(http) TXT
GSE130274_TS1015_normalized_counts_DESeq2_20190417_renamed.txt.gz 3.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap