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Series GSE13075 Query DataSets for GSE13075
Status Public on Nov 26, 2008
Title SIRT1 and H1AcK26 promoter-association in mouse ES cells changes upon oxidative stress
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by array
Summary Association of the (histone) deacetylase SIRT1 with promoters was assessed to determine putative SIRT1-regulated genes. Based on the observation that the SIRT1 yeast ortholog Sir2 redistributes across the yeast genome in repsonse to genotoxic stress and double strand breaks (DSBs), we investigated the impact of oxidative stress on the chromatin binding pattern of SIRT1. Oxidative stress causes a major change in SIRT1 binding that is accompanied by an inverse H1K26 acetylation pattern. H1K26 was shown to be a direct target for deacetylation by SIRT1.

Keywords: ChIP-chip, stress response
Overall design ES cells were left untreated or treated with 2 mM H2O2 for 1 hour. Both samples were subjected to ChIP against SIRT1, H1AcK26 or rabbit Ig (one IP per treatment and Ab). Input DNA was labeled with Cy3, IP DNA with Cy5, enrichment over input is reported as 2log.
Contributor(s) Oberdoerffer P, Sinclair DA
Citation(s) 19041753
Submission date Oct 07, 2008
Last update date Mar 20, 2012
Contact name Philipp Oberdoerffer
Organization name Harvard Medical School
Department Pathology
Lab Sinclair
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL7496 NimbleGen 1.5 kb mouse promoter ChIP-chip (version 2005-03-31_MM5_min_promoter)
Samples (5)
GSM334239 ES cells_untreated_SIRT1-IP
GSM334240 ES cells_untreated_H1AcK26-IP
GSM334241 ES cells_untreated_Rabbit-Ig-IP
This SubSeries is part of SuperSeries:
GSE13121 SIRT1 redistribution on chromatin promotes genome stability but alters gene expression during aging
BioProject PRJNA114267

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Supplementary file Size Download File type/resource
GSE13075_RAW.tar 86.0 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table

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