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Series GSE130826 Query DataSets for GSE130826
Status Public on May 31, 2021
Title Effect of Cell Division on Cell Differentiation (ChIP-Seq HUvsNoc)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We addressed the role of cell division during differentiation by blocking the progression of the cell cycle during mesendoderm differentiation. Human pluripotent stem cells (H9 cells) were synchronized in their cell cycle and then induced to differentiate into mesendoderm for 24h. To block the cells in their S phase, we added Hydroxyurea (200 uM) at the beginning of the differentiation process. To block the cells in their G2/M phases, we added nocodazole (100 ng/ml) 8h after the induction of differentiation. At the end of the experiment, the cells were harvested and processed for RNA extraction.
Overall design Four different conditions were analyzed: Undifferentiated (UD), Mesendoderm (E24), Mesendoderm + Hydroxyurea (E24HU), and Mesendoderm + nocodazole (E24Noc). Two biological replicates were generated for each condition.
Contributor(s) Grandy R
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Submission date May 07, 2019
Last update date May 31, 2021
Contact name Rodrigo Andres Grandy
Organization name University of Cambridge
Department Surgery
Lab Vallier Lab
Street address Cambridge Stem Cell Institute/ Anne McLaren Laboratory West Forvie Building, Robinson Way
City Cambridge
ZIP/Postal code CB2 0SZ
Country United Kingdom
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (24)
GSM3754931 Input_UDR1
GSM3754932 Input_E24R1
GSM3754933 Input_E24HUR1
This SubSeries is part of SuperSeries:
GSE130830 Effect of Cell Division on Cell Differentiation
BioProject PRJNA541527
SRA SRP195816

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Supplementary file Size Download File type/resource
GSE130826_GRCh38_gencode.gtf.gz 39.0 Mb (ftp)(http) GTF 988.4 Mb (ftp)(http) BW 862.7 Mb (ftp)(http) BW 968.4 Mb (ftp)(http) BW 906.8 Mb (ftp)(http) BW 907.2 Mb (ftp)(http) BW 951.4 Mb (ftp)(http) BW 982.6 Mb (ftp)(http) BW 921.2 Mb (ftp)(http) BW 892.6 Mb (ftp)(http) BW 799.6 Mb (ftp)(http) BW 932.0 Mb (ftp)(http) BW 896.1 Mb (ftp)(http) BW 868.3 Mb (ftp)(http) BW 829.3 Mb (ftp)(http) BW 877.4 Mb (ftp)(http) BW 891.9 Mb (ftp)(http) BW
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