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| Status |
Public on Oct 30, 2019 |
| Title |
Blue Light Sensing in Listeria monocytogenes Is Temperature-Dependent and the Transcriptional Response to It Is Predominantly SigB-Dependent |
| Organism |
Listeria monocytogenes |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study the global transcriptional response of L. monocytogenes to blue light was elucidated using an RNAseq-based approach. A transcriptomic analysis of the response to sub-lethal levels of blue light found that the changes in transcription were almost entirely SigB-dependent. A mutant where the light sensing mechanism of RsbL was inactivated through an amino acid substitution (Cys56Ala) was found to have an attenuated response to blue light, but residual activation of SigB-dependent genes suggested that alternative routes for activation of SigB by light are likely to exist.
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| |
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| Overall design |
Comparison between wild-type and mutant cells cultured in darkness and in light.
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| |
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| Contributor(s) |
Dorey AL, O'Byrne CP, Lee B, Rotter B |
| Citation(s) |
31798538 |
| |
| Submission date |
May 09, 2019 |
| Last update date |
Jan 30, 2020 |
| Contact name |
Amber Louise Dorey |
| E-mail(s) |
amber.dorey@nuigalway.ie
|
| Organization name |
National University of Ireland Galway
|
| Department |
Microbiology
|
| Lab |
Bacterial Stress Response Group
|
| Street address |
University Road
|
| City |
Galway |
| State/province |
Galway |
| ZIP/Postal code |
H91 CF50 |
| Country |
Ireland |
| |
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| Platforms (1) |
| GPL26477 |
Illumina NextSeq 500 (Listeria monocytogenes) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA542147 |
| SRA |
SRP200048 |
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