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Series GSE131633 Query DataSets for GSE131633
Status Public on Aug 22, 2019
Title RNA sequencing of bone marrow WT and SLAMF9-/- pDCs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary SLAMF9 belongs to the conserved lymphocytic activation molecule family (SLAMF). Unlike other SLAMs, which have been extensively studied, the role of SLAMF9 in the immune system remained mostly unexplored. By generating CRISPR/CAS9 SLAMF9 knockout mice, we analyzed the role of this receptor in plasmacytoid dendritic cells (pDCs), which preferentially express the SLAMF9 transcript and protein. These cells display a unique capacity to produce type I interferon and bridge between innate and adaptive immune response. Analysis of pDCs in SLAMF9-/- mice revealed an increase of immature pDCs in the BM and enhanced accumulation of pDCs in the lymph nodes. In the periphery, SLAMF9 deficiency resulted in lower levels of the transcription factor SPIB, elevation of pDC survival, and attenuated IFNand TNFproduction. To define the role of SLAMF9 during inflammation, pDCs lacking SLAMF9 were followed during induced experimental autoimmune encephalomyelitis (EAE). SLAMF9-/- mice demonstrated attenuated disease and delayed onset, accompanied by a prominent increase of immature pDCs in the LN, with a reduced co-stimulatory potential and enhanced infiltration of pDCs into the central nervous system (CNS). These results suggest the crucial role of SLAMF9 in pDC differentiation, homeostasis and function in the steady state and during EAE.
Overall design Comparing the transcriptom of pDCs derived from WT and SLAMF9-/- mice
Contributor(s) Sever L, Shachar I, Friedlander G
Citation(s) 31346085
Submission date May 22, 2019
Last update date Aug 22, 2019
Contact name Gilgi Friedlander
Organization name Weizmann Institute of Science
Street address Hertzel st
City Rehovot
ZIP/Postal code 76100
Country Israel
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM3791482 Wild type replica1
GSM3791483 Wild type replica2
GSM3791484 Wild type replica3
BioProject PRJNA544261
SRA SRP199215

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Supplementary file Size Download File type/resource
GSE131633_DEoutput.xlsx 2.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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