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Series GSE131826 Query DataSets for GSE131826
Status Public on Jun 16, 2020
Title RNA-binding Protein PCBP1/hnRNP E1 is an Intracellular Checkpoint for Balancing Effector versus Regulatory T Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Distinct lineages of T cells can act in response to various environmental cues to either drive or restrict immune-mediated pathology. Immunotherapy involving the block of cell surface immune checkpoints CTLA-4 and PD-1/PD-L1 has revolutionized cancer treatment across a wide spectrum of malignancies. However, additional immune regulatory mechanisms remain to be discovered, and will facilitate development of next generation agents to overcome resistance to current therapies. We now report that the RNA-binding protein, PCBP1, is critical for stabilizing effector T cell functions by limiting expression of T effector cell-intrinsic Treg-commitment programs and subverting expression of immune-suppressive signals. Indeed, T cell-specific deletion of Pcbp1 increased Treg development, enlisted multiple inhibitory checkpoint molecules including PD-1, TIGIT and VISTA on TILs, and thwarted anti-tumor immunity. Our results demonstrate a critical role for PCBP1 as an intracellular checkpoint for balancing effector versus Treg cells and also suggest PCBP1 as a novel target for cancer immunotherapy.
Overall design Spleen mRNA profiles of 6-8 weeks old wild type (WT) and Pcbp1f/fCD4-Cre male mice were generated by deep sequencing, in quadruplicate, using Illumina HiSeq3000.
Contributor(s) Chung D, Ansa-Addo E
Citation(s) 32523987
Submission date May 28, 2019
Last update date Jun 16, 2020
Contact name Zihai Li
Organization name Ohio State University
Department Institute for Immuno-Oncology
Street address 460 W 12th Ave
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (8)
GSM3820326 E1KO rep1
GSM3820327 E1KO rep2
GSM3820328 E1KO rep3
BioProject PRJNA545087
SRA SRP199625

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131826_RAW.tar 810.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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