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Series GSE132068 Query DataSets for GSE132068
Status Public on Sep 21, 2019
Title Phenotypic characterisation of regulatory T cells in dogs reveals signature transcripts conserved in humans and mice
Organism Canis lupus
Experiment type Expression profiling by high throughput sequencing
Summary Regulatory T cells (Tregs) are a double-edged regulator of the immune system. Aberrations of Tregs correlate with pathogenesis of inflammatory, autoimmune and neoplastic disorders. Phenotypically and functionally distinct subsets of Tregs have been identified in humans and mice on the basis of their extensive portfolios of monoclonal antibodies (mAb) against Treg surface antigens. As an important veterinary species, dogs are increasingly recognised as an excellent model for many human diseases. However, insightful study of canine Tregs has been restrained by the limited availability of mAb. We therefore set out to characterise CD4+CD25high T cells isolated ex vivo from healthy dogs and showed that they possess a regulatory phenotype, function, and transcriptomic signature that resembles those of human and murine Tregs. By launching a cross-species comparison, we unveiled a conserved transcriptomic signature of Tregs and identified that transcript hip1 may have implications in Treg function.
 
Overall design Transcriptomic profiles of CD4+CD25high and CD4+CD25- T cells isolated ex vivo from blood of five healthy dogs were generated by RNA-seq, using Illumina HiSeq 4000 System.
Web link https://rdcu.be/bRix4
 
Contributor(s) Garden OA, Wu Y
Citation(s) 31530890
Submission date Jun 02, 2019
Last update date Sep 21, 2019
Contact name Ying Wu
E-mail(s) juliawuu@vet.upenn.edu
Organization name University of Pennsylvania
Street address 3900 Spruce Street
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL26727 Illumina HiSeq 4000 (Canis lupus)
Samples (10)
GSM3842065 CD4+CD25high_1
GSM3842066 CD4+CD25high_2
GSM3842067 CD4+CD25high_3
Relations
BioProject PRJNA545835
SRA SRP200110

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132068_GAR3761A1-13_tpm_gene_count_table.txt.gz 1.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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