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Status |
Public on Sep 21, 2019 |
Title |
Phenotypic characterisation of regulatory T cells in dogs reveals signature transcripts conserved in humans and mice |
Organism |
Canis lupus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Regulatory T cells (Tregs) are a double-edged regulator of the immune system. Aberrations of Tregs correlate with pathogenesis of inflammatory, autoimmune and neoplastic disorders. Phenotypically and functionally distinct subsets of Tregs have been identified in humans and mice on the basis of their extensive portfolios of monoclonal antibodies (mAb) against Treg surface antigens. As an important veterinary species, dogs are increasingly recognised as an excellent model for many human diseases. However, insightful study of canine Tregs has been restrained by the limited availability of mAb. We therefore set out to characterise CD4+CD25high T cells isolated ex vivo from healthy dogs and showed that they possess a regulatory phenotype, function, and transcriptomic signature that resembles those of human and murine Tregs. By launching a cross-species comparison, we unveiled a conserved transcriptomic signature of Tregs and identified that transcript hip1 may have implications in Treg function.
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Overall design |
Transcriptomic profiles of CD4+CD25high and CD4+CD25- T cells isolated ex vivo from blood of five healthy dogs were generated by RNA-seq, using Illumina HiSeq 4000 System.
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Web link |
https://rdcu.be/bRix4
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Contributor(s) |
Garden OA, Wu Y |
Citation(s) |
31530890 |
Submission date |
Jun 02, 2019 |
Last update date |
Sep 21, 2019 |
Contact name |
Ying Wu |
E-mail(s) |
juliawuu@vet.upenn.edu
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Organization name |
University of Pennsylvania
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Street address |
3900 Spruce Street
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City |
Philadelphia |
State/province |
Pennsylvania |
ZIP/Postal code |
19104 |
Country |
USA |
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Platforms (1) |
GPL26727 |
Illumina HiSeq 4000 (Canis lupus) |
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Samples (10)
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Relations |
BioProject |
PRJNA545835 |
SRA |
SRP200110 |