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| Status |
Public on Jul 04, 2019 |
| Title |
Transcriptome Analysis of IL-33-Deficient Early Developing B Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified that IL-33 is expressed in pro-B (B220+ IgM- CD19+ CD43+) and large pre-B cells (B220+ IgM- CD19+ CD43- FSC-Ahi SSC-Ahi) in mice. We assessed for IL-33-dependent gene expression differences in these B cell stages by performing bulk RNA-sequencing of FACS-purified pro-B and large pre-B cells from WT and IL-33-deficient 8 week old adult, naive female mice.
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| |
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| Overall design |
Examination of poly-A enriched transcriptome of WT and IL-33-deficient pro-B and large pre-B cells.
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| |
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| Contributor(s) |
Stokes Peebles R, Eischen C |
| Citation(s) |
31391233 |
| |
| Submission date |
Jun 03, 2019 |
| Last update date |
Oct 03, 2019 |
| Contact name |
Matthew Stier |
| E-mail(s) |
stiermt@gmail.com
|
| Organization name |
Vanderbilt University Medical Center
|
| Department |
Internal Medicine
|
| Lab |
B1227 MCN
|
| Street address |
1161 21st Avenue South
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA545985 |
| SRA |
SRP200258 |
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