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| Status |
Public on Jun 20, 2019 |
| Title |
Activation Dynamics and Immunoglobulin Evolution of Pre-existing and Newly Generated Human Memory B-cell Responses to Influenza Hemagglutinin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Understanding human vaccine-induced memory B cell responses is critical to developing effective vaccines to evolving viruses like Influenza A. B cell responses to influenza exposure involve activation of pre-existing immunity and generation of new responses. To characterize these two types of responses, we analyzed the response to H7N9 vaccination in H7N9 naïve adults. This allowed us to distinguish responses of pre-existing B cells towards conserved epitopes versus newly generated H7-specific epitopes using hemagglutinin (HA) probes by flow cytometry. The recall response to conserved epitopes on H7 HA involved a transient expansion of memory B cells with little observed adaptation. However, the B cell response to newly encountered epitopes was phenotypically distinct and generated a sustained memory population that evolved and affinity matured months after vaccination. These findings establish clear differences between newly generated and pre-existing memory B cells, highlighting the challenges in achieving long-lasting, broad protection against an ever-evolving virus.
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| Overall design |
34 Samples
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| Contributor(s) |
Andrews SF, Chambers MJ, Schramm CA, Plyler J, Raab JE, Kanekiyo M, Gillespie RA, Ransier A, Darko S, Hu J, Chen X, Yassine HM, Boyington JC, Crank MC, Chen GL, Coates C, Mascola JR, Douek DC, Graham BS, Ledgerwood JE, McDermott AB |
| Citation(s) |
31350180 |
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| Submission date |
Jun 18, 2019 |
| Last update date |
Sep 19, 2019 |
| Contact name |
Chaim A Schramm |
| Organization name |
NIAID, NIH
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| Department |
Vaccine Research Center
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| Lab |
Genome Analysis Core
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| Street address |
40 Convent Dr
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA549473 |
| SRA |
SRP201769 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE132923_RAW.tar |
17.5 Mb |
(http)(custom) |
TAR (of CSV) |
| GSE132923_README.txt |
733.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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