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Series GSE133166 Query DataSets for GSE133166
Status Public on Jan 07, 2020
Title Collaborative interactions of heterogenous ribonucleoproteins contribute to transcriptional regulation of sterol metabolism
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a group of functionally versatile proteins that play critical roles in the biogenesis, cellular localization and transport of RNA. Although several lines of evidence link hnRNP abnormalities to neurodegenerative diseases and cancer their contribution to metabolic control remains unexplored. Here, we outline a role for hnRNPs in regulatory circuits controlling sterol homoeostasis. Specifically, we find that tissue-selective loss of the conserved hnRNP RALY enriches for metabolic pathways arguing that hnRNPs can discriminately influence regulated gene expression programs. Liver-specific deletion of RALY alters hepatic lipid content and serum cholesterol level. In vivo interrogation of chromatin architecture and genome-wide RALY binding pattern reveal insights into its cooperative interactions and mode of action in regulating cholesterogenesis. Interestingly, we find that RALY binds the promoter region of the master metabolic regulator Srebp2 and show that it directly interacts with coactivator NFY to influence cholesterogenic gene expression. Our work offers new insights into mechanisms orchestrating selective promoter activation in metabolic control and a model by which hnRNPs can impact metabolic health and disease states.
 
Overall design RALY KO and histone-DNA complexes
 
Contributor(s) Zhang Z, Sallam T
Citation(s) 32080181
Submission date Jun 21, 2019
Last update date Mar 09, 2020
Contact name Zhengyi Zhang
E-mail(s) zhengyizhang@mednet.ucla.edu
Organization name UCLA
Street address Westwood
City Los Angeles
ZIP/Postal code 90055
Country USA
 
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (19)
GSM3901298 RNA-seq_WT_Rep1
GSM3901299 RNA-seq_WT_Rep2
GSM3901300 RNA-seq_WT_Rep3
Relations
BioProject PRJNA550126
SRA SRP202553

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133166_RAW.tar 1.8 Gb (http)(custom) TAR (of BEDGRAPH)
GSE133166_Raly_RNA-seq_count.txt.gz 888.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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