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Series GSE133174 Query DataSets for GSE133174
Status Public on Oct 28, 2020
Title Ascending colon-derived mucin-type O-glycans form key mucus layers encapsulating microbiota in the colon (RNA-seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The colon contains a dense metabolically potent microbiota. The colonic O-glycan-rich mucus has been recognized as a key barrier to prevent microbial intrusion, but how this system forms and functions remains unclear. Here, we discovered that the colon mucus is mainly forged by microbiota-dependent secretion of O-glycosylated Muc2 by goblet cells in the ascending colon, where it seamlessly encapsulates the fecal materials including the microbiota. Deletion of O-glycans in the ascending colon impaired the segregating function of the mucous coating, leading to altered structure and metabolic output of the microbiota, and transcriptional homeostasis of the entire host mucosa. These findings represent a paradigm change of the prevailing model of the colon mucus system and provides new insights into host and microbiota symbiosis.
Overall design RNA seq was performed on mouse colon tissue derived from the ascending and descending colon. Global gene expression profiles were compared between 3 Groups of Healthy Control and O-glycan mutant littermates that were all colonized with the same microbiota
Contributor(s) Bergstrom K, Shan X, Casero D, Batushansky A, Lagishetty V, Jacobs JP, Hoover C, McDaniel JM, McGee S, Houchen CW, Rao C, Griffin T, Sonnenburg JL, McEver RP, Braun J, Xia L
Citation(s) 33093110
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 DK085691 Role of Mucin-type O-glycans in Intestinal Inflammation OKLAHOMA MEDICAL RESEARCH FOUNDATION Lijun Xia
Submission date Jun 21, 2019
Last update date Jan 27, 2021
Contact name David Casero
Phone 3108250274
Organization name UCLA
Department Pathology and Laboratory Medicine
Street address 610 Charles Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (30)
GSM3901363 Group1-Desc-Control-1
GSM3901364 Group1-Desc-Control-2
GSM3901365 Group1-Desc-TMDKOAsc-1
This SubSeries is part of SuperSeries:
GSE133257 Ascending colon-derived mucin-type O-glycans form key mucus layers encapsulating microbiota in the colon
BioProject PRJNA550137
SRA SRP202556

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Supplementary file Size Download File type/resource
GSE133174_ProDisExpression.genesfpkm.txt.gz 995.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
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