NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE133334 Query DataSets for GSE133334
Status Public on Jul 09, 2019
Title Sleep Deprivation Alters the Pituitary Stress Transcriptome in male and female Mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed a comparative, whole-transcriptome, analysis to identify stress-induced genes and relevant pathways that may be affected by sleep deprivation.
Methods: One day following 12 hours of Paradoxical Sleep Deprivation (PSD), mice were restrained for 20 minutes. Gene expression changes in the pituitary were assessed via RNA-Seq and Gene Ontology in PSD and/or restrained groups compared to controls.
Results: We show that restraint triggers transcriptional responses involved in hormone secretion, the glucocorticoid response, and apoptosis in both sexes, with 285 differentially expressed genes in females and 93 in males. When PSD preceded restraint stress, the numbers of differentially expressed genes increased to 613 in females and 580 in males. The pituitary transcriptome of restraint+PSD animals was enriched for microglia and macrophage proliferation, cellular response to corticosteroids, and apoptosis, among others. Finally, we show sex-specific differences in restraint-induced genes following PSD.
Conclusion: The results indicate striking differences in the male and female stress-induced transcriptome, as well as in the PSD-induced changes. When PSD preceded the restraint stress challenge, the effects on the pituitary transcriptome were striking. While the male and female PSD + restraint-induced transcriptome was similar, we detected remarkable differences, perhaps indicating different strategies used by each sex to cope with challenges to homeostasis. We hope that these data illuminate future research elucidating how sleep deprivation impacts the vital response to stress and motivate the analysis of male and female subjects when designing experiments.
 
Overall design Gene expression changes in the pituitary were assessed via RNA-Seq and Gene Ontology in Paradoxical Sleep Deprivation and/or restrained groups compared to controls.
 
Contributor(s) Oyola MG, Shupe E, Soltis AR, Sukumar G, Paez-Pereda M, Larco DO, Wilkerson M, Rothwell S, Dalgard CL, Wu TJ
Citation(s) 31649619
Submission date Jun 26, 2019
Last update date Oct 31, 2019
Contact name Mario G Oyola
Organization name Uniformed Services University
Street address 4301 Jones Bridge Rd
City Bethesda
State/province MD
ZIP/Postal code 20814
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (28)
GSM3905891 PHN-101f
GSM3905892 PHN-101m
GSM3905893 PHN-102f
Relations
BioProject PRJNA551143
SRA SRP212107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133334_sample_gene_counts.txt.gz 1.1 Mb (ftp)(http) TXT
GSE133334_sample_gene_normalized_counts.txt.gz 2.9 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap