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Series GSE133532 Query DataSets for GSE133532
Status Public on Jun 29, 2019
Title BR-body mutant mRNA half-life profiling.
Organism Caulobacter vibrioides NA1000
Experiment type Expression profiling by high throughput sequencing
Summary BR-body mutant strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. JS38 (wild type) was compared to JS221 (lacking the intrinsically disordered CTD and unable to form BR-bodies), JS233 (unable to recruit degradosome components into BR-bodies), and JS299 (active site mutant of RNase E).
 
Overall design BR-body mutant strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. Biological samples were taken before treatment, then at 1, 3, and 9 minutes after rifampicin treatment.
 
Contributor(s) Schrader JM
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Submission date Jun 28, 2019
Last update date Jul 07, 2019
Contact name Jared Michael Schrader
E-mail(s) schrader@wayne.edu
Organization name Wayne State University
Department Biological Sciences
Lab Schrader Lab
Street address 5047 Gullen Mall, Biological Sciences Building Room 2168
City Detroit
State/province MI
ZIP/Postal code 48202
Country USA
 
Platforms (1)
GPL26862 Illumina HiSeq 4000 (Caulobacter vibrioides NA1000)
Samples (16)
GSM3911381 JS38 0 min
GSM3911382 JS38 1 min
GSM3911383 JS38 3 min
Relations
BioProject PRJNA551694
SRA SRP212634

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Supplementary file Size Download File type/resource
GSE133532_Half-life_processed_data.txt.gz 169.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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