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| Status |
Public on Nov 21, 2008 |
| Title |
High-throughput pyrosequencing of endogenous small RNAs associated to human Argonaute 1 & 2 |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Small non-coding RNAs function in concert with Argonaute (Ago) proteins to regulate gene expression at the level of transcription, mRNA stability or translation. Ago proteins bind small RNAs and form the core of silencing complexes. Here we report the analysis of small RNAs associated with human Ago1 and Ago2 revealed by immunoprecipitation and deep sequencing. Among the reads we find small RNAs originating from the small nucleolar RNA (snoRNA) ACA45. Moreover, processing of ACA45 requires Dicer activity but is independent of Drosha/DGCR8. Using bio-informatic prediction algorithms and luciferase reporter assays, we uncover the mediator subunit CDC2L6 as one potential mRNA target of ACA45 small RNAs suggesting a role for ACA45 processing products in post-transcriptional gene silencing. We further identify a number of human snoRNAs with microRNA (miRNA)- like processing signatures. We have therefore identified a novel class of small RNAs in human cells that originate from snoRNAs and can function like miRNAs.
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| Overall design |
Two samples examined. Small RNAs associated to human Argonaute 1 and human Argonaute 2
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| |
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| Contributor(s) |
Ender C, Krek A, Friedländer MR, Beitzinger M, Rajewsky N, Meister G |
| Citation(s) |
19026782 |
| |
| Submission date |
Oct 27, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
Gunter Meister |
| E-mail(s) |
meister@biochem.mpg.de
|
| Organization name |
Max Planck Institute of Biochemistry
|
| Department |
Center for Integrated Protein Science Munich (CIPSM)
|
| Lab |
RNA Biology
|
| Street address |
Am Klopferspitz 18
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
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| Platforms (1) |
|
| Samples (2) |
| GSM337570 |
endogenous small RNA associated to human Argonaute 1 |
| GSM337571 |
endogenous small RNA associated to human Argonaute 2 |
|
| Relations |
| SRA |
SRP001364 |
| BioProject |
PRJNA109793 |
