|
| Status |
Public on Dec 22, 2023 |
| Title |
Role of MITF in advanced lethal prostate cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Great efforts have been made to identify key molecular aberrations that sustain growth and confer resistance to androgen deprivation therapy (ADT) in advanced prostate cancer (PC), and yet PC remains a lethal disease. Recent years have witnessed the discovery of several master regulator transcription factors that enhance lethal PC aggressiveness and provide actionable targets that may improve patient survival. Here we explore the role of the microphthalmia transcription factor (MITF) in lethal prostate cancer. To identify the mechanisms through which MITF mododulates prostate cancer aggressiveness, we knock-down MITF in three prostate cancer cell lines to identify the MITF regulated effector gene network contributing to lethal prostate cancer.
Methods: We compared global transcription of three prostate cancer cell lines transduced with a siRNA control and 2 siRNAs targetting MITF by RNAseq.
Results: RNA-seq of MITF knockdown prostate cancer cells uncovered a trasncriptional network of MITF regulated genes
Conclusions: MITF regulates a discrette gene network that contributes to prostate cancer aggressiveness
|
| |
|
| Overall design |
To characterize the transcriptional program regulated by MITF, we performed RNA sequencing of three PC models, DU145, 22Rv1 and ARCaPM, after 48 hours of being transfected with siRNA control or two siRNAs targeting MITF (biological replicates of n = 3 for each condition).
|
| |
|
| Contributor(s) |
Hassan A, Domingo-Domenech J, Hoshida Y |
| Citation(s) |
37676710 |
| |
| Submission date |
Jul 08, 2019 |
| Last update date |
Dec 22, 2023 |
| Contact name |
Yujin Hoshida |
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (27)
|
|
| Relations |
| BioProject |
PRJNA553232 |
| SRA |
SRP213716 |
Less...
More...