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Series GSE134037 Query DataSets for GSE134037
Status Public on Jun 06, 2021
Title Single-cell analysis of whole hepatic non-parenchymal cells from normal and CCl4-injured mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary This study used 10X Genomics, single-cell RNA-sequencing to examine the heterogeneity of liver non-parenchymal cell types present in the normal and CCl4-mediated fibrotic mice.
Overall design Two male mice of 8-week old were injected i.p with olive oil and CCl4 independently twice a week for a total 6 weeks. Then, liver non-parenchymal cells were obtained by a two-step collagenase perfusion method. Hepatocytes were elimanated by three times of low-speed centrifugation at 50g for three minutes and were not included in further analysis of this study. Single-cell suspensions were then loaded on a Chromium Single Cell Instrument (10X Genomics) to generate single-cell GEMs. After reverse transcription, libraries were prepared using version 3 Chromium Single-cell 3' Library, Gel Bead & Mutiplex Kit (10X Genomics). Sequencing was performed on illumina HiSeq 3000.
Contributor(s) Ruan B, Wang L
Citation(s) 34277612, 35114417
Submission date Jul 09, 2019
Last update date May 05, 2022
Contact name Bai Ruan
Organization name Fourth Military Medical University
Street address No.169, Changle Road
City Xi'an
State/province Shannxi
ZIP/Postal code 710032
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (2)
GSM3933661 Q
GSM3933662 F
BioProject PRJNA553529
SRA SRP213889

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Supplementary file Size Download File type/resource
GSE134037_RAW.tar 37.9 Mb (http)(custom) TAR (of XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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