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Series GSE134079 Query DataSets for GSE134079
Status Public on Dec 02, 2019
Title Comparative transcriptome analysis of melatonin or auxin treated-Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Objective: Melatonin and auxin are both tryptophan-derived indole molecules. Much attention has been given to proposed auxin-like activities of melatonin (regulating growth concentration-dependently). However, it still largely remains unclear whether melatonin and auxin regulate signalling pathways in a similar fashion. The purpose of this study is to directly compare the transcriptome response of Arabidopsis with melatonin or auxin.
Method: mRNA profiles of 12-day old rosettes treated for a further of 3 days with +/- melatonin (5µM, 1005µM) or NAA(4.5µM) were generated by RNA-sequencing in triplicates (three independent biological experiments), using Illumina NextSeq 550 technology.
Results: Comparative global transcriptome analysis conducted on Arabidopsis rosette treated with melatonin or NAA under exact same set of experimental conditions revealed differential number of genes and type of pathways. While auxin (4.5µM) regulated a large number of genes and elicited a diverse response, melatonin (100µM) showed a modest effect on transcriptome with only few genes significantly regulated whereas none regulated at approximately equimolar concentration with NAA (5µM) as compared to untreated solvent control (0.1% EtOH). Interestingly, the most prominent category of genes regulated by melatonin trended towards biotic stress defense pathways.
Conclusions: These findings indicate that melatonin and auxin act quite differently toward signaling pathways in Arabidopsis. Melatonin has its own set of mechanisms to exert its functions, with strong inclination toward biotic defense pathways.
Overall design mRNA profiles of +/- melatonin (5µM or 100µM) or NAA(4.5 µM)-treated Arabidopsis rosettes were generated by RNA-sequencing in triplicates (three independent biological experiments), using Illumina NextSeq 550.
Contributor(s) Zia SF, Bedon F, Berkowitz O, Whelan J, Franks AE, Plummer KM
Citation(s) 31856719
Submission date Jul 10, 2019
Last update date Jan 08, 2020
Contact name Sajal Fatima Zia
Organization name La Trobe University
Department Animal, Plant and Soil Sciences
Lab Kim Plummer
Street address Agribio, 5 Ring Rd, Bundoora VIC 3083
City Melbourne
State/province VIC
ZIP/Postal code 3083
Country Australia
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (12)
GSM3936256 0.1% EtOH rep1
GSM3936258 0.1% EtOH rep2
GSM3936259 0.1% EtOH rep3
BioProject PRJNA553702
SRA SRP214076

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Supplementary file Size Download File type/resource
GSE134079_processed_data_file_TPM.csv.gz 1.0 Mb (ftp)(http) CSV
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Processed data are available on Series record

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