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Series GSE13427 Query DataSets for GSE13427
Status Public on Nov 15, 2008
Title A toolbox for epitope-tagging and genome-wide location analysis in Candida albicans
Organism Candida albicans
Experiment type Genome binding/occupancy profiling by array
Summary Background : Candida albicans is a diploid pathogenic fungus not yet amenable to routine genetic investigations. Understanding aspects of the regulation of its biological functions and the assembly of its protein complexes would lead to further insight into the biology of this common disease-causing microbial agent.
Results: We have developed a toolbox allowing in vivo protein tagging by PCR-mediated homologous recombination with TAP, HA and MYC tags. The transformation cassettes were designed to accommodate a common set of integration primers. The tagged proteins can be used to perform tandem affinity purification (TAP) or chromatin immunoprecipitation coupled with microarray analysis (ChIP-CHIP). Tandem affinity purification of C. albicans Nop1 revealed the high conservation of the small processome composition in yeasts. Data obtained with in vivo TAP-tagged Tbf1, Cbf1 and Mcm1 recapitulates previously published genome-wide location profiling by ChIP-CHIP. We also designed a new reporter system for in vivo analysis of transcriptional activity of gene loci in C. albicans.
Conclusion: This toolbox provides a basic setup to perform purification of protein complexes and increase the number of annotated transcriptional regulators and genetic circuits in C. albicans.
Overall design Two independent biological replicates of ChIP-CHIP of Mcm1-TAP in yeast and hyphal states. ChIP-CHIP of Cbf1-TAP and Tbf1-TAP.
Contributor(s) Lavoie H, Sellam A, Askew C, Nantel A, Whiteway M
Citation(s) 19055720
Submission date Oct 31, 2008
Last update date Mar 20, 2012
Contact name Hugo Lavoie
Phone 514-496-6154
Fax 514-496-6213
Organization name CNRC-IRB
Department Genetics
Street address 6100 Royalmount
City Montreal
State/province Quebec
ZIP/Postal code H4P 2R2
Country Canada
Platforms (1)
GPL6474 Candida albicans ChIP-CHIP array
Samples (6)
GSM338661 ChIP-CHIP of Cbf1-TAP
GSM338662 ChIP-CHIP of Tbf1-TAP
GSM338663 ChIP-CHIP of Mcm1-TAP in yeast state
BioProject PRJNA110007

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13427_RAW.tar 7.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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