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Series GSE134466 Query DataSets for GSE134466
Status Public on Feb 04, 2021
Title INPP4B protects from metabolic disease and obesity associated prostate neoplasia.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A high fat diet and obesity have been linked to the development of metabolic dysfunction and the promotion of multiple cancers in mice and men. The causative cellular signals are multifactorial and not completely understood. Previous studies have shown that metabolic dysfunction leads to activation of the AKT and PKC pathways. Both signaling pathways are inhibited by the dual specificity phosphatase, INPP4B, which dephosphorylates PI(3,4)P2, an activator of AKT, and PI(4,5)P2, an activator of the PLC/PKC pathway. We established that INPP4B signaling protects mice from metabolic dysfunction. Inpp4b-/- male mice had accelerated activation of SREBF1 in liver which, along with the high fat diet, caused increased expression and activity of PPARG and other lipogenic pathways leading non-alcoholic fatty liver disease (NAFLD), type II diabetes, expansion and inflammation of WAT, and systemic and localized prostate inflammation that drives the development of high-grade prostatic intraepithelial neoplasia (PIN).
 
Overall design WT and Inpp4b-/- males were fed with either LFD (LabDiet 5V75, St. Louis, MO) or HFD (TestDiet 58R3, St. Louis, MO). The HFD consisted of 59.4% fat, 25.7% carbohydrate, and 14.9% protein (total 22.8 kJ/g), whereas the regular chow contained 12.9% fat, 63.8% carbohydrate, and 23.2% protein (total 13.6 kJ/g). All LFD WT, LFD Inpp4b-/-, HFD WT, and HFD Inpp4b-/- mice were euthanized and dissected at 12 weeks and their tissues where collected for analysis. RNA was purified from HFD WT (N=4) and HFD Inpp4b-/- (N = 4) and submitted to Novogene for RNA sequencing.
 
Contributor(s) Zhang M, Kaftanovskaya EY, Vasquez JL, Vacher J, Nathanson L, Agoulnik AI, Ittmann MM, Agoulnik IU
Citation(s) 33772116
Submission date Jul 18, 2019
Last update date Apr 20, 2021
Contact name Lubov Nathanson
E-mail(s) lnathanson@nova.edu
Organization name Nova Southeastern University
Department INIM
Street address 3321 College Ave.
City Fort Lauderdale
State/province Florida
ZIP/Postal code 33314
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM3949159 Inpp4b-/- rep1 [IN_1648]
GSM3949160 Inpp4b-/- rep2 [IN_1649]
GSM3949161 Inpp4b-/- rep3 [IN_1675]
Relations
BioProject PRJNA555198
SRA SRP215197

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134466_HISAT2_forDS.csv.gz 285.6 Kb (ftp)(http) CSV
GSE134466_norm_countsH.csv.gz 954.7 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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