|
| Status |
Public on Jul 08, 2020 |
| Title |
Disruption of TFIIH activities generates a transcriptional stress gene expression response and reveals possible new targets against cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The global effect on transcription between cancer cells and their progenitors and investigating how these compounds affect TFIIH integrity. Here, we used an oncogenesis model to compare the effect of TFIIH inhibitors: TPL and THZ1 between transformed cells and their progenitors. RNA-seq and RNAPII-ChIP-seq experiments showed that although the levels of many transcripts were reduced, the levels of a significant number were increased after TPL treatment, with maintained or increased RNAPII promoter occupancy
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| |
|
| Overall design |
RNAseq in cells MCF10A-Er-Src with TPL 4h-125 nM and RNAPII-ChIP-seq with TPL 4h-125 nM
|
| |
|
| Contributor(s) |
Uriostegui-Arcos M, Zurita M |
| Citation(s) |
32543350, 38250805 |
| |
| Submission date |
Aug 01, 2019 |
| Last update date |
Feb 09, 2024 |
| Contact name |
Mario Zurita |
| E-mail(s) |
marioz@ibt.unam.mx
|
| Organization name |
UNAM
|
| Street address |
Av. Universidad 2001
|
| City |
Cuernavaca |
| State/province |
Morelos |
| ZIP/Postal code |
62250 |
| Country |
Mexico |
| |
|
| Platforms (2) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (18)
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|
| Relations |
| BioProject |
PRJNA558076 |
| SRA |
SRP217234 |
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