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Series GSE135861 Query DataSets for GSE135861
Status Public on Apr 01, 2020
Title Transcriptome analysis of methylparaben treated H1299 lung carcinoma cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: finding differential transcripts usage in environmentally harzardous chemical, methylparaben
Methods: transcriptome analysis by the RNA-seq via mRNA pull-down
Results: We found that methylparaben increases cellular toxicity in H1299 cells. Also, over thousands of retained intron events were detected from the RNA-seq analysis.
Conclusions: Methylparaben increases cytotoxic effects in H1299 cells through the caspase-3 pathway and changes the context of trascript through the splicing regulation mechanisms.
 
Overall design H1299 cells were treated with methylparaben and all experiments were duplicated.
 
Contributor(s) Kim J
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Submission date Aug 15, 2019
Last update date Apr 01, 2020
Contact name Jung-Woong Kim
E-mail(s) jungkim@cau.ac.kr
Organization name Chung-Ang University
Department Life Science
Lab Genome Dynamics Lab
Street address 84 Heuksuk-ro Dongjak-ku
City Seoul
ZIP/Postal code 06974
Country South Korea
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM4036764 H1299 cells, EtOH rep1
GSM4036765 H1299 cells, EtOH rep2
GSM4036766 H1299 cells, Methyl-paraben rep1
Relations
BioProject PRJNA560363
SRA SRP218423

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135861_normalized_counts_FPKM_.txt.gz 8.0 Mb (ftp)(http) TXT
GSE135861_raw_counts.txt.gz 733.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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