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Status |
Public on May 21, 2020 |
Title |
RNA-seq analysis for E.coli dnd and dam derivatives |
Organisms |
Escherichia coli K-12; Escherichia coli BW25113 |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To get a global view of PT modification’s function in ∆dam mutant, we did an RNA-seq to compare the gene expression change after dam knocks out and test whether PT could restore the changes.
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Overall design |
We selected mid-log-phase cultures of E.coli ∆dam mutant with or without expressing dndBCDE, to perform RNAseq analysis with wild-type E.coli BW25113 as the reference.
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Contributor(s) |
Wu X, Chen C, Jiang S, Wang L, Chen S, Dedon PC |
Citation missing |
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Submission date |
Aug 18, 2019 |
Last update date |
May 24, 2020 |
Contact name |
Xiaolin Wu |
E-mail(s) |
xiaolinwu@whu.edu.cn
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Organization name |
Singapore-MIT Alliance for Research and Technology (SMART)
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Street address |
1 CREATE Way
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City |
Singapore |
State/province |
Singapore |
ZIP/Postal code |
138602 |
Country |
Singapore |
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Platforms (2) |
GPL24377 |
Illumina HiSeq 4000 (Escherichia coli K-12) |
GPL25146 |
Illumina HiSeq 4000 (Escherichia coli BW25113) |
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Samples (3) |
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This SubSeries is part of SuperSeries: |
GSE135949 |
Epigenetic competition reveals density-dependent regulation and target site plasticity of phosphorothioate epigenetics in bacteria |
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Relations |
BioProject |
PRJNA560689 |
SRA |
SRP218682 |