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Series GSE136045 Query DataSets for GSE136045
Status Public on Aug 11, 2021
Title Acclimation of rice leaves to varying temperatures
Organism Oryza sativa
Experiment type Expression profiling by high throughput sequencing
Summary The extent to which light-saturated net CO2 assimilation (An) and leaf dark respiratory CO2 release (Rdark) jointly acclimate to abrupt and sustained changes in temperature (T) in rice (Oryza sativa L.) is unclear, as are the underlying mechanisms associated with thermal acclimation. To further our understanding of how sustained changes in temperature affect the carbon economy of rice, hydroponically-grown plants of the IR64 cultivar were developed at 30/25°C (day/night) in a temperature-controlled greenhouse before being shifted to 25/20°C or 40/35°C. Leaf RNA expression, protein abundance, sugar and starch content, gas-exchange and elongation rates were measured on pre-existing leaves (PE) already developed at 30/25°C, or leaves newly-developed (ND) subsequent to temperature transfer.
Overall design To investigate changes in gene expression following temperature shifts in rice we employed total RNA-seq capturing both poly(A) and non-poly(A) transcripts. Rice plants were grown under control conditions at day/night temperatures of 30/25 °C (”warm”) for 2 weeks, then shifted to either 40/35 °C “hot” or 25/20 °C “cold” treatments. Samples were then collected for RNA-seq at 0 hr, 2 hr, 6 hr and 24 hr time points (n = 4).
Contributor(s) Crisp PA, Scafaro A, Berkowitz O, Whelan J, Atkin OK
Citation(s) 31860752
Submission date Aug 20, 2019
Last update date Nov 15, 2021
Contact name Peter A Crisp
Organization name The University of Queensland
Department School of Agriculture and Food Science
Lab Crisp
Street address John Hines Building (62), The University of Queensland
City Brisbane
State/province QLD
ZIP/Postal code 4072
Country Australia
Platforms (1)
GPL25149 Illumina HiSeq 1500 (Oryza sativa)
Samples (40)
GSM4040027 cold treated 2 hr replicate 1 [C1-2hr_S29_L003]
GSM4040028 cold treated 2 hr replicate 2 [C2-2hr_S32_L004]
GSM4040029 cold treated 2 hr replicate 3 [C3-2hr_S35_L004]
BioProject PRJNA561097
SRA SRP218873

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Supplementary file Size Download File type/resource
GSE136045_rice_temp_RNAseq_seq_voom_normalied_gene_expression.csv.gz 6.4 Mb (ftp)(http) CSV
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