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Series GSE136119 Query DataSets for GSE136119
Status Public on Aug 22, 2019
Title MeCP2 regulates gene expression through direct interaction with H3K27me3 [Targeted_BS_seq: DMSO_vs_GSK343]
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary MeCP2 plays a multifaceted role in gene expression regulation and chromatin organization. Interaction between MeCP2 and methylated DNA to regulate gene expression is well established. However, the widespread MeCP2 distribution suggests its additional interactions with chromatin. Here we show, by both biochemical and ChIP-seq analyses, that MeCP2 directly binds to nucleosome subunit proteins and is recruited to distinct chromatin regions where H3K27me3 is enriched. We further observed that the impact of MeCP2 on transcriptional changes is correlated with histone post-translational modification patterns. Our findings indicate that MeCP2 can be recruited to genomic loci via indirect binding and that interaction between MeCP2 and histone proteins plays a significant role in gene expression regulation.
 
Overall design To analyze the effect of GSK343 ‚Äč‚Äčtreatment on DNA methylation, targeted bisulfite sequencing was performed using the SureSelectXT Methyl-Seq Target Enrichment System of Illumina Multiplexed Sequencing (Agilent Technologies).
Web link https://pubmed.ncbi.nlm.nih.gov/32561780/
 
Contributor(s) Lee W, Gong Q
Citation(s) 32561780
Submission date Aug 21, 2019
Last update date Jun 23, 2020
Contact name Wooje Lee
E-mail(s) ntinamu001@gmail.com
Organization name Chosun University
Department Cellular and Molecular Medicine
Street address 309, Pilmun-daero, Dong-gu
City Gwangju
ZIP/Postal code 61452
Country South Korea
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM4041354 Targeted_Bisulfite_DMSO_rep1
GSM4041355 Targeted_Bisulfite_DMSO_rep2
GSM4041356 Targeted_Bisulfite_GSK343_rep1
This SubSeries is part of SuperSeries:
GSE122366 MeCP2 regulates genome-wide gene expression through recognition of H3K27me3
Relations
BioProject PRJNA561340
SRA SRP218991

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136119_RAW.tar 16.6 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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