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Series GSE13614 Query DataSets for GSE13614
Status Public on Jun 02, 2009
Title Expression profiling of the forming atrioventricular node using a novel Tbx3-based node-specific transgenic reporter
Organism Mus musculus
Experiment type Expression profiling by array
Summary The atrioventricular (AV) node is a recurrent source of potentially life-threatening arrhythmias. Nevertheless, limited data are available on its developmental control or molecular phenotype. We used a novel AV node-specific reporter mouse to gain insight into the gene programs determining the formation and phenotype of the AV node. In the transgenic reporter, green fluorescent protein (GFP) expression was driven by 160 kbp of Tbx3 and flanking sequences. GFP was selectively expressed in the AV canal of embryos, and in the AV node of adults, while all other Tbx3+ conduction system components, including the AV bundle, were devoid of GFP expression. Fluorescent AV nodal (Tbx3BAC-Egfp) and complementary working (NppaBAC336-Egfp) myocardial cell populations of E10.5 embryos and E17.5 fetuses were purified using fluorescence-activated cell sorting, and their expression profiles were assessed by microarray analysis. We constructed a comprehensive list of sodium, calcium, and potassium channels specific for the nodal or working myocard. Furthermore, the data revealed that the AV node and the working myocardium phenotypes diverge during development, but that the functional gene classes characteristic for both compartments are maintained. Interestingly, the AV node-specific gene repertoire consisted of multiple neurotrophic factors not yet appreciated to play a role in nodal development. These data present the first genome-wide transcription profiles of the AV node during development, providing valuable information concerning its molecular identity.

Keywords: Tbx3, AV node, working myocardium, embryonic development, cardiac development, cardiac conduction system
Overall design 24 samples:
6x working myocardium stage E10.5 (NppaBAC336-Egfp mice),
6x AV canal myocardium stage E10.5 (Tbx3BAC-Egfp mice),
6x working myocardium stage E17.5 (NppaBAC336-Egfp mice),
6x AV node myocardium stage E17.5 (Tbx3BAC-Egfp mice)
Contributor(s) Horsthuis T, Buermans HP, Brons JF, Verkerk AO, Wakker V, Clout DE, Bakker ML, Moorman AF, ’t Hoen PA, Christoffels VM
Citation(s) 19498200
Submission date Nov 14, 2008
Last update date Jan 18, 2013
Contact name Henk Buermans
Phone +31715268558
Organization name Leiden University Medical Center
Department Human Genetics
Lab Leiden Genome Technology Center
Street address Albinusdreef 2
City Leiden
State/province ZH
ZIP/Postal code 2300 RC
Country Netherlands
Platforms (1)
GPL6105 Illumina mouse-6 v1.1 expression beadchip
Samples (24)
GSM343145 working myocardium _ stage e10.5 _ biorep1
GSM343146 AV canal myocardium_ stage e10.5 _ biorep1
GSM343147 working myocardium _ stage e10.5 _ biorep2
BioProject PRJNA110637

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13614_RAW.tar 5.2 Mb (http)(custom) TAR
GSE13614_unprocessed_intensity_values.txt 7.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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