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Series GSE136174 Query DataSets for GSE136174
Status Public on Aug 20, 2022
Title Molecular mechanisms underlying zinc oxide nanoparticle induced insulin resistance in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Zinc oxide nanoparticles (ZnO NPs) represent an important class of commercially applied materials. Recently, adverse effects of ZnO NPs were found in humans and animals following ingestion, although the effects on endocrine system disease remain unclear. In this study, ZnO NPs were orally administered to mice, and at doses of 25 mg/kg bw (body weight) ZnO NPs and above, plasma glucose increased significantly. The genome-wide effects of ZnO NPs were then investigated using RNA-sequencing technology. In the cluster analysis, the most significantly enriched Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways concerned membranes and their close association with endoplasmic reticulum (ER) stress and reactive oxygen species (ROS) generation. Biochemical and gene and protein expression analyses revealed that ZnO NPs activated a xenobiotic biodegradation response and increased the expression of cytochrome P450 (CYP) enzymes in mice livers, leading to ER stress. The ER stress increased ROS generation. The high levels of ROS activated the MAPK and NF-B pathways and induced an inflammation response, resulting in the phosphorylation of insulin receptor substrate 1. Thus, the insulin resistance that developed was the primary mechanism for the increase in the plasma glucose of mice treated orally with ZnO NPs.
Overall design Examination of genome-wide expressions in Control, Week8 and Week 12 groups
Contributor(s) Gu N, Hu H
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Submission date Aug 22, 2019
Last update date Aug 22, 2022
Contact name Hailong Hu
Phone 13019726056
Organization name Harbin Institute of Technology
Street address No.2 of Yikuang Road, 2E-215
City Harbin
ZIP/Postal code 150001
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (3)
GSM4042110 Sample1: Control
GSM4042111 Sample2: Week8
GSM4042112 Sample3: Week12
BioProject PRJNA561500
SRA SRP219073

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Supplementary file Size Download File type/resource
GSE136174_RAW.tar 9.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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