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Series GSE136177 Query DataSets for GSE136177
Status Public on Jun 10, 2021
Title A chemical and synthetic biology approach to control plant membrane receptor kinase signaling
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Plants have a large family of membrane receptor kinases (RKs) which sense extracellular signals to control plant growth, development, immunity, and stress response. The largest group of RKs contains an extracellular leucine-rich repeat (LRR) domain with over 200 members in Arabidopsis. However, the functional understanding of most of the LRR-RKs has been hampered by their genetic redundancy and the subtle phenotypes of RK overexpression. Here we show that the rapamycin-mediated heterodimerization of chimeric cytosolic kinase domains from receptor/co-receptor pairs in the plasma membrane can activate their downstream cellular signaling pathway, inducing the specific biological responses, including brassinosteroid, plant immunity, stomatal development, and lateral root development. This chemically controlled synthetic biology approach will be useful to investigate biological functions of LRR-RKs and their signaling pathways.
 
Overall design Arabidopsis seedlings were grown on medium containing 2 μM PPZ with 100 nM brassinolide or 100 nM rapamycin in the dark for 5 days before harvesting for total RNA extraction.
 
Contributor(s) Oh E
Citation(s) 33964457
Submission date Aug 22, 2019
Last update date Jun 10, 2021
Contact name Sara Kim
E-mail(s) 8462sara@gmail.com
Organization name Korea university
Department Life sciences and biotechnology
Lab plant environment interaction lab
Street address 서울 성북구 안암동5가 1-2 고려대학교 안암캠퍼스 하나과학관 505호
City seoul
ZIP/Postal code 02841
Country South Korea
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (10)
GSM4042148 CM1
GSM4042149 CM2
GSM4042150 CR1
Relations
BioProject PRJNA561509
SRA SRP219081

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136177_Processed.txt.gz 1.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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