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Series GSE136203 Query DataSets for GSE136203
Status Public on Sep 19, 2020
Title RNA sequencing of the stomachs of H. pylori-infected WT and Mincle–/– mice after 8 weeks
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Helicobacter pylori (H. pylori) causes assorted gastrointestinal disorders. H. pylori-specific T cells develop during infection, which is a prerequisite for the induction of gastritis and malignancy. How the innate immune system senses H. pylori to prime T cells remains unclear. We report that cholesteryl glucosides in H. pylori activate innate immunity through C-type lectin receptors (CLRs). Cholesteryl acyl α-glucoside (αCAG) was identified as a ligand for Mincle (Clec4e). Upon H. pylori infection, T cell responses and gastritis were ameliorated in Mincle-deficient mice, although bacterial numbers were comparable.Thus the cholesteryl lipid–CLR axis exacerbates H. pylori-induced gastric inflammation with limited contribution to protective immunity.
 
Overall design RNAseq analysis was performed by using RNA extracted from the stomachs of H. pylori-infected WT and Mincle–/– mice after 8 weeks in triplicates.
 
Contributor(s) Nagata M, Okuzaki D, Yamasaki S
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Submission date Aug 22, 2019
Last update date Sep 21, 2020
Contact name Daisuke Okuzaki
E-mail(s) dokuzaki@biken.osaka-u.ac.jp
Phone +81-6-6879-4935
Organization name Osaka univ.
Department Immunology Frontier Research Center
Lab Human Immunology (Single Cell Genomics)
Street address Yamadaoka 3-1
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (6)
GSM4042557 infected_wt_stomach_rep1
GSM4042558 infected_wt_stomach_rep2
GSM4042559 infected_wt_stomach_rep3
Relations
BioProject PRJNA561590
SRA SRP219102

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136203_RAW.tar 800.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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